The Cytokeratin 8 Polyclonal Antibody (CAB24434) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene.
This antibody is validated for use in WB applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Cytokeratin 8 Polyclonal Antibody
SKU:
CAB24434
Size:
100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WB
Recommended Dilution:
WB
,1:500 - 1:1000
Synonyms:
K8, KO, CK8, CK-8, CYK8, K2C8, CARD2, 8
Positive Sample:
HeLa, Mouse large intestine
Cellular Localization:
Cytoplasm, Nucleus, Nucleus Matrix, Nucleoplasm.
Calculated MW:
54 kDa/56 kDa
Observed MW:
54 kDa
This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene.
Purification Method
Affinity purification
Gene ID
3856
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse large intestine, using Cytokeratin 8 Rabbit pAb (CAB24434) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of lysates from wild type(WT) and Cytokeratin 8 knockout (KO) HeLa cells, using Cytokeratin 8 Rabbit pAb (CAB24434) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 0.5s.
