Anti-DCP1A Antibody (CAB7376)

Antibody Name:	Anti-DCP1A Antibody
Antibody SKU:	CAB7376
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 303-582 of human DCP1A (NP_060873.4).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:20 - 1:50
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, Jurkat, Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 303-582 of human DCP1A (NP_060873.4).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	NEKH APTY TIPL SPVL SPTL PAEA PTAQ VPPS LPRN STMM QAVK TTPR QRSP LLNQ PVPE LSHA SLIA NQSP FRAP LNVT NTAG TSLP SVDL LQKL RLTP QHDQ IQTQ PLGK GAMV ASFS PAAG QLAT PESF IEPP SKTA AARV AASA SLSN MVLA PLQS MQQN QDPE VFVQ PKVL SSAI PVAG APLV TATT TAVS SVLL APSV FQQT VTRS SDLE RKAS SPSP LTIG TPES QRKP SIIL SKSQ LQDT LIHL IKND SSFL STLH EVYL QVLT KNKD NHNL
Gene ID:	55802
Uniprot:	Q9NPI6
Cellular Location:	Cytoplasm, Nucleus, P-body
Calculated MW:	59kDa/63kDa
Observed MW:	70KDa

Synonyms:

DCP1A, HSA275986, Nbla00360, SMAD4IP1, SMIF

Background:

Decapping is a key step in general and regulated mRNA decay. The protein encoded by this gene is a decapping enzyme. This protein and another decapping enzyme form a decapping complex, which interacts with the nonsense-mediated decay factor hUpf1 and may be recruited to mRNAs containing premature termination codons. This protein also participates in the TGF-beta signaling pathway. Alternative splicing of this gene results in multiple transcript variants.

UniProt Protein Function:	DCP1A: a decapping enzyme. Plays a role in general and regulated mRNA decay. Apparently plays a role in the nonsense-mediated decay pathway that rids cells of mRNAs containing premature termination codons. May function as a transactivator of SMAD4 that participates in TGF-beta signaling.
UniProt Protein Details:	Protein type:RNA processing; Transcription, coactivator/corepressor; EC 3.-.-.-; Hydrolase Chromosomal Location of Human Ortholog: 3p21.1 Cellular Component: cytosol; intracellular membrane-bound organelle; membrane; nucleus Molecular Function:enzyme activator activity; enzyme regulator activity; hydrolase activity; identical protein binding; mRNA binding; protein binding Biological Process: deadenylation-dependent decapping; deadenylation-independent decapping; gene expression; mRNA catabolic process, deadenylation-dependent decay; mRNA catabolic process, nonsense-mediated decay; positive regulation of catalytic activity; regulation of mRNA stability
NCBI Summary:	Decapping is a key step in general and regulated mRNA decay. The protein encoded by this gene is a decapping enzyme. This protein and another decapping enzyme form a decapping complex, which interacts with the nonsense-mediated decay factor hUpf1 and may be recruited to mRNAs containing premature termination codons. This protein also participates in the TGF-beta signaling pathway. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Feb 2014]
UniProt Code:	Q9NPI6
NCBI GenInfo Identifier:	296434475
NCBI Gene ID:	55802
NCBI Accession:	Q9NPI6.2
UniProt Secondary Accession:	Q9NPI6,B4DHN9, U3KQM8,
UniProt Related Accession:	Q9NPI6
Molecular Weight:	59,361 Da
NCBI Full Name:	mRNA-decapping enzyme 1A
NCBI Synonym Full Names:	decapping mRNA 1A
NCBI Official Symbol:	DCP1A
NCBI Official Synonym Symbols:	SMIF; SMAD4IP1; HSA275986; Nbla00360
NCBI Protein Information:	mRNA-decapping enzyme 1A
UniProt Protein Name:	mRNA-decapping enzyme 1A
UniProt Synonym Protein Names:	Smad4-interacting transcriptional co-activator; Transcription factor SMIF
Protein Family:	mRNA-decapping enzyme
UniProt Gene Name:	DCP1A
UniProt Entry Name:	DCP1A_HUMAN

	Western blot analysis of extracts of various cell lines, using DCP1A Rabbit pAb (CAB7376) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of A549 cells using DCP1A antibody (CAB7376). Blue: DAPI for nuclear staining.

Antibodies

Anti-DCP1A Antibody (CAB6824)

DCP1A Antibody (PACO01516)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)