Anti-DCP2 Antibody (CAB8282)

Antibody Name:	DCP2 Rabbit Polyclonal Antibody
Antibody SKU:	CAB8282
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human DCP2 (NP_001229306.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HT-29, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human DCP2 (NP_001229306.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	METK RVEI PGSV LDDL CSRF ILHI PSEE RDNA IRVC FQIE LAHW FYLD FYMQ NTPG LPQC GIRD FAKA VFSH CPFL LPQG EDVE KVLD EWKE YKMG VPTY GAII LDET LENV LLVQ GYLA KSGW GFPK GKVN KEEA PHDC AARE VFEE TGFD IKDY ICKD DYIE LRIN DQLA RLYI IPGI PKDT KFNP KTRR EIRN IEWF SIEK LPCH RNDM TPKS KLGL APNK FFMA IPFI RPLR DWLS
Gene ID:	167227
Uniprot:	Q8IU60
Cellular Location:	Cytoplasm, Nucleus, P-body
Calculated MW:	44kDa/48kDa
Observed MW:	50kDa

Synonyms:	DCP2, NUDT20
Background:	The protein encoded by this gene is a key component of an mRNA-decapping complex required for degradation of mRNAs, both in normal mRNA turnover, and in nonsense-mediated mRNA decay (NMD). It removes the 7-methyl guanine cap structure from mRNA, prior to its degradation from the 5' end. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

UniProt Protein Function:	DCP2: Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Plays a role in replication-dependent histone mRNA degradation. Removes the 7- methyl guanine cap structure from mRNA molecules, yielding a 5'- phosphorylated mRNA fragment and 7m-GDP. Has higher activity towards mRNAs that lack a poly(A) tail. Has no activity towards a cap structure lacking a RNA moiety. Belongs to the Nudix hydrolase family. DCP2 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 3.6.1.62; Hydrolase; RNA processing Chromosomal Location of Human Ortholog: 5q22.2 Cellular Component: cell junction; cytosol; intracellular membrane-bound organelle; nucleoplasm Molecular Function:exoribonuclease activity, producing 5'-phosphomonoesters; m7G(5')pppN diphosphatase activity; protein binding Biological Process: deadenylation-dependent decapping; mRNA catabolic process; mRNA catabolic process, nonsense-mediated decay; regulation of mRNA stability
NCBI Summary:	The protein encoded by this gene is a key component of an mRNA-decapping complex required for degradation of mRNAs, both in normal mRNA turnover, and in nonsense-mediated mRNA decay (NMD). It removes the 7-methyl guanine cap structure from mRNA, prior to its degradation from the 5' end. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Jun 2011]
UniProt Code:	Q8IU60
NCBI GenInfo Identifier:	269849560
NCBI Gene ID:	167227
NCBI Accession:	Q8IU60.2
UniProt Secondary Accession:	Q8IU60,Q6P2D4, Q7Z5W5, Q8NBG5, C9J778,
UniProt Related Accession:	Q8IU60
Molecular Weight:	44,443 Da
NCBI Full Name:	m7GpppN-mRNA hydrolase
NCBI Synonym Full Names:	decapping mRNA 2
NCBI Official Symbol:	DCP2
NCBI Official Synonym Symbols:	NUDT20
NCBI Protein Information:	m7GpppN-mRNA hydrolase
UniProt Protein Name:	m7GpppN-mRNA hydrolase
UniProt Synonym Protein Names:	Nucleoside diphosphate-linked moiety X motif 20; Nudix motif 20; mRNA-decapping enzyme 2; hDpc
Protein Family:	mRNA-decapping enzyme
UniProt Gene Name:	DCP2
UniProt Entry Name:	DCP2_HUMAN

	Western blot analysis of extracts of various cell lines, using DCP2 antibody (CAB8282) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse lung using DCP2 antibody (CAB8282) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat kidney using DCP2 antibody (CAB8282) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver damage using DCP2 antibody (CAB8282) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using DCP2 antibody (CAB8282) at dilution of 1:100. Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)