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Anti-DDAH1 Antibody (CAB10295)

SKU:
CAB10295
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
  • Cell Biology Antibodies 1 Anti-DDAH1 Antibody CAB10295
  • Cell Biology Antibodies 1 Anti-DDAH1 Antibody CAB10295
€139 - €419
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Description

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Antibody Name:Anti-DDAH1 Antibody
Antibody SKU:CAB10295
Antibody Size:20uL, 50uL, 100uL
Application:WB IF IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-285 of human DDAH1 (NP_036269.1).
Application:WB IF IP
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:SW480, 22Rv1, HT-29, 293T, SGC-7901, Mouse brain, Mouse kidney, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-285 of human DDAH1 (NP_036269.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAGL GHPA AFGR ATHA VVRA LPES LGQH ALRS AKGE EVDV ARAE RQHQ LYVG VLGS KLGL QVVE LPAD ESLP DCVF VEDV AVVC EETA LITR PGAP SRRK EVDM MKEA LEKL QLNI VEMK DENA TLDG GDVL FTGR EFFV GLSK RTNQ RGAE ILAD TFKD YAVS TVPV ADGL HLKS FCSM AGPN LIAI GSSE SAQK ALKI MQQM SDHR YDKL TVPD DIAA NCIY LNIP NKGH VLLH RTPE EYPE SAKV YEKL KDHM LIPV SMSE LEKV DGLL TCCS VLIN KKVD S
Gene ID:23576
Uniprot:O94760
Cellular Location:
Calculated MW:20kDa/31kDa
Observed MW:37kDa
Synonyms:DDAH1, DDAH, DDAH-1, DDAHI, HEL-S-16
Background:This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity.
UniProt Protein Function:DDAH1: Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation. Belongs to the DDAH family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 3.5.3.18; Hydrolase

Chromosomal Location of Human Ortholog: 1p22.3

Cellular Component: cytosol

Molecular Function:catalytic activity; dimethylargininase activity

Biological Process: citrulline metabolic process; nitric oxide mediated signal transduction; positive regulation of nitric oxide biosynthetic process; regulation of nitric-oxide synthase activity; regulation of systemic arterial blood pressure

NCBI Summary:This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. [provided by RefSeq, Jul 2008]
UniProt Code:O94760
NCBI GenInfo Identifier:6831528
NCBI Gene ID:23576
NCBI Accession:O94760.3
UniProt Secondary Accession:O94760,Q5HYC8, Q86XK5,
UniProt Related Accession:O94760
Molecular Weight:31kDa
NCBI Full Name:N(G),N(G)-dimethylarginine dimethylaminohydrolase 1
NCBI Synonym Full Names:dimethylarginine dimethylaminohydrolase 1
NCBI Official Symbol:DDAH1  
NCBI Official Synonym Symbols:DDAH; DDAHI; DDAH-1; HEL-S-16  
NCBI Protein Information:N(G),N(G)-dimethylarginine dimethylaminohydrolase 1
UniProt Protein Name:N(G),N(G)-dimethylarginine dimethylaminohydrolase 1
UniProt Synonym Protein Names:DDAHI; Dimethylargininase-1
Protein Family:N(G),N(G)-dimethylarginine dimethylaminohydrolase
UniProt Gene Name:DDAH1  
Anti-DDAH1 Antibody (CAB10295)Western blot analysis of extracts of various cell lines, using DDAH1 antibody (CAB10295) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-DDAH1 Antibody (CAB10295)Immunofluorescence analysis of NIH-3T3 cells using DDAH1 Polyclonal Antibody (CAB10295) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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