Western blot analysis of Mouse brain, using DDR1/MCK10/CD167a antibody (CAB22705) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The DDR1/MCK10/CD167a Monoclonal Antibody (CAB22705) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed through mouse hybridoma technology, is highly specific and reliable for detecting DDR1 in various biological samples. It has been validated for use in immunofluorescence, immunoprecipitation, and flow cytometry applications.DDR1 is a receptor tyrosine kinase involved in cellular processes such as cell proliferation, differentiation, and survival.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
DDR1/MCK10/CD167a Monoclonal Antibody
SKU:
CAB22705
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Clone Number:
ARC58637
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Cell Membrane, Secreted, Single-Pass Type I Membrane Protein.
Calculated MW:
101kDa
Observed MW:
125kDa
Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. The protein encoded by this gene belongs to a subfamily of tyrosine kinase receptors with homology to Dictyostelium discoideum protein discoidin I in their extracellular domain, and that are activated by various types of collagen. Expression of this protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Purification Method
Affinity purification
Gene ID
780
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using DDR1/MCK10/CD167a Rabbit mAb (CAB22705) at 1:1000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): Mouse liver Exposure time: 60s.
