Anti-DDX17 Antibody (CAB17078)

Antibody Name:	Anti-DDX17 Antibody
Antibody SKU:	CAB17078
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of human DDX17.

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SKOV3, HT-29, Mouse ovary, Mouse brain

Immunogen:	Recombinant protein of human DDX17.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	10521
Uniprot:	Q92841
Cellular Location:
Calculated MW:	72kDa/80kDa
Observed MW:	80kDa

Synonyms:

DDX17, P72, RH70

Background:

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and splicesosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an ATPase activated by a variety of RNA species, but not by dsDNA. This protein, and that encoded by DDX5 gene, are more closely related to each other than to any other member of the DEAD box family. This gene can encode multiple isoforms due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) start codon.

UniProt Protein Function:	DDX17: RNA-dependent ATPase activity. Involved in transcriptional regulation. Transcriptional coactivator for estrogen receptor ESR1. Increases ESR1 AF-1 domain-mediated transactivation. Synergizes with DDX5 and SRA1 RNA to activate MYOD1 transcriptional activity and probably involved in skeletal muscle differentiation. Required for zinc-finger antiviral protein ZC3HAV1-mediated mRNA degradation. Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:RNA processing; RNA-binding; Nucleolus; Spliceosome; Transcription, coactivator/corepressor; EC 3.6.4.13; Nuclear receptor co-regulator; Helicase Chromosomal Location of Human Ortholog: 22q13.1 Cellular Component: nucleoplasm; membrane; nucleolus; nucleus Molecular Function:protein binding; RNA-dependent ATPase activity; RNA binding; transcription coactivator activity; estrogen receptor binding; RNA helicase activity; ATP binding Biological Process: RNA processing; transcription, DNA-dependent; positive regulation of estrogen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter
NCBI Summary:	DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and splicesosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an ATPase activated by a variety of RNA species, but not by dsDNA. This protein, and that encoded by DDX5 gene, are more closely related to each other than to any other member of the DEAD box family. This gene can encode multiple isoforms due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) start codon. [provided by RefSeq, Apr 2011]
UniProt Code:	Q92841
NCBI GenInfo Identifier:	380865374
NCBI Gene ID:	10521
NCBI Accession:	Q92841.2
UniProt Secondary Accession:	Q92841,Q69YT1, Q6ICD6, B1AHM0,
UniProt Related Accession:	Q92841
Molecular Weight:	729
NCBI Full Name:	Probable ATP-dependent RNA helicase DDX17
NCBI Synonym Full Names:	DEAD (Asp-Glu-Ala-Asp) box helicase 17
NCBI Official Symbol:	DDX17
NCBI Official Synonym Symbols:	P72; RH70
NCBI Protein Information:	probable ATP-dependent RNA helicase DDX17; DEAD box protein p72; RNA-dependent helicase p72; DEAD (Asp-Glu-Ala-Asp) box polypeptide 17; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 17 (72kD)
UniProt Protein Name:	Probable ATP-dependent RNA helicase DDX17
UniProt Synonym Protein Names:	DEAD box protein 17; DEAD box protein p72; RNA-dependent helicase p72
Protein Family:	Probable ATP-dependent RNA helicase
UniProt Gene Name:	DDX17
UniProt Entry Name:	DDX17_HUMAN

	Western blot analysis of extracts of various cell lines, using DDX17 antibody (CAB17078) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5min.
	Immunofluorescence analysis of U2OS cells using DDX17 antibody (CAB17078) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Anti-DDX17 Antibody (CAB5731)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)