Anti-DDX31 Antibody (CAB15892)

Antibody Name:	Anti-DDX31 Antibody
Antibody SKU:	CAB15892
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 652-851 of human DDX31 (NP_073616.6).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 652-851 of human DDX31 (NP_073616.6).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EIKM EDIL CVLT RDDC FKGK RWGA QKSH AVGP QEIR ERAT VLQT VFED YVHS SERR VSWA KKAL QSFI QAYA TYPR ELKH IFHV RSLH LGHV AKSF GLRD APRN LSAL TRKK RKAH VKRP DLHK KTQS KHSL AEIL RSEY SSGM EADI AKVK KQNA PGEP GGRP LQHS LQPT PCFG RGKT LKWR KTQK GVQR DSKT SQKV
Gene ID:	64794
Uniprot:	Q9H8H2
Cellular Location:	Nucleus, nucleolus
Calculated MW:	34kDa/64kDa/85kDa/94kDa
Observed MW:	94kDa

Synonyms:

DDX31, PPP1R25

Background:

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates multiple transcript variants encoding different isoforms.

UniProt Protein Function:	DDX31: Probable ATP-dependent RNA helicase. Belongs to the DEAD box helicase family. DDX31/DBP7 subfamily. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 3.6.4.13; Hydrolase; Nucleolus Chromosomal Location of Human Ortholog: 9q34.13 Cellular Component: Golgi apparatus; intracellular membrane-bound organelle; nucleolus Molecular Function:ATP binding; ATP-dependent RNA helicase activity; protein binding Biological Process: ribosome biogenesis and assembly; RNA secondary structure unwinding
NCBI Summary:	DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9H8H2
NCBI GenInfo Identifier:	17505907
NCBI Gene ID:	64794
NCBI Accession:	NP_073616.6
UniProt Secondary Accession:	Q9H8H2,Q5K6N2, Q5K6N3, Q5K6N4, Q5VZJ4, Q5VZJ9, Q96E91 Q96NY2, Q96SX5, Q9H5K6,
UniProt Related Accession:	Q9H8H2
Molecular Weight:	64,137 Da
NCBI Full Name:	probable ATP-dependent RNA helicase DDX31 isoform 1
NCBI Synonym Full Names:	DEAD-box helicase 31
NCBI Official Symbol:	DDX31
NCBI Official Synonym Symbols:	PPP1R25
NCBI Protein Information:	probable ATP-dependent RNA helicase DDX31
UniProt Protein Name:	Probable ATP-dependent RNA helicase DDX31
UniProt Synonym Protein Names:	DEAD box protein 31; Helicain
Protein Family:	Probable ATP-dependent RNA helicase
UniProt Gene Name:	DDX31
UniProt Entry Name:	DDX31_HUMAN

	Western blot analysis of extracts of A-549 cells, using DDX31 antibody (CAB15892) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min.
	Immunofluorescence analysis of HeLa cells using DDX31 antibody (CAB15892) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using DDX31 antibody (CAB15892) at dilution of 1:100. Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)