Anti-DDX3Y Antibody (CAB11704)

Antibody Name:	Anti-DDX3Y Antibody
Antibody SKU:	CAB11704
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human DDX3Y (NP_001116137.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, B cells, 293T, A-431, HeLa, Mouse testis, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human DDX3Y (NP_001116137.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSHV VVKN DPEL DQQL ANLD LNSE KQSG GAST ASKG RYIP PHLR NREA SKGF HDKD SSGW SCSK DKDA YSSF GSRD SRGK PGYF SERG SGSR GRFD DRGR SDYD GIGN RERP GFGR FERS GHSR WCDK SVED DWSK PLPP SERL EQEL FSGG NTGI NFEK YDDI PVEA TGSN CPPH IENF SDID MGEI IMGN IELT RYTR PTPV QKHA IP
Gene ID:	8653
Uniprot:	O15523
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	32kDa/72kDa/73kDa
Observed MW:	73kDa

Synonyms:

DDX3Y, DBY

Background:

The protein encoded by this gene is a member of the DEAD-box RNA helicase family, characterized by nine conserved motifs, included the conserved Asp-Glu-Ala-Asp (DEAD) motif. These motifs are thought to be involved in ATP binding, hydrolysis, RNA binding, and in the formation of intramolecular interactions. This protein shares high similarity to DDX3X, on the X chromosome, but a deletion of this gene is not complemented by DDX3X. Mutations in this gene result in male infertility, a reduction in germ cell numbers, and can result in Sertoli-cell only sydrome. Pseudogenes sharing similarity to both this gene and the DDX3X paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	DDX3Y: a Y-linked DEAD box protein. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. DBY mutations causes male infertility, Sertoli cell-only syndrome or severe hypospermatogenesis, suggesting that this gene plays a key role in the spermatogenic process. Two alternatively spliced transcripts differ only in the length of the 3' UTR.
UniProt Protein Details:	Protein type:RNA-binding; EC 3.6.4.13; Helicase Chromosomal Location of Human Ortholog: Yq11 Cellular Component: cytoplasm; membrane Molecular Function:ATP-dependent RNA helicase activity Biological Process: chromosome segregation; regulation of gene expression; RNA secondary structure unwinding; translational initiation Disease: Spermatogenic Failure, Y-linked, 2
NCBI Summary:	The protein encoded by this gene is a member of the DEAD-box RNA helicase family, characterized by nine conserved motifs, included the conserved Asp-Glu-Ala-Asp (DEAD) motif. These motifs are thought to be involved in ATP binding, hydrolysis, RNA binding, and in the formation of intramolecular interactions. This protein shares high similarity to DDX3X, on the X chromosome, but a deletion of this gene is not complemented by DDX3X. Mutations in this gene result in male infertility, a reduction in germ cell numbers, and can result in Sertoli-cell only sydrome. Pseudogenes sharing similarity to both this gene and the DDX3X paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]
UniProt Code:	O15523
NCBI GenInfo Identifier:	73620957
NCBI Gene ID:	8653
NCBI Accession:	O15523.2
UniProt Secondary Accession:	O15523,Q8IYV7, B4DK29, B4DXX7,
UniProt Related Accession:	O15523
Molecular Weight:	32,416 Da
NCBI Full Name:	ATP-dependent RNA helicase DDX3Y
NCBI Synonym Full Names:	DEAD-box helicase 3, Y-linked
NCBI Official Symbol:	DDX3Y
NCBI Official Synonym Symbols:	DBY
NCBI Protein Information:	ATP-dependent RNA helicase DDX3Y
UniProt Protein Name:	ATP-dependent RNA helicase DDX3Y
UniProt Synonym Protein Names:	DEAD box protein 3, Y-chromosomal
Protein Family:	ATP-dependent RNA helicase
UniProt Gene Name:	DDX3Y
UniProt Entry Name:	DDX3Y_HUMAN

	Western blot analysis of extracts of various cell lines, using DDX3Y antibody (CAB11704) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded rat testis using DDX3Y antibody (CAB11704) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using DDX3Y antibody (CAB11704) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using DDX3Y antibody (CAB11704) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using DDX3Y antibody (CAB11704) at dilution of 1:100 (40x lens).

Antibodies

Anti-DDX3Y Antibody (CAB14810)

DDX3Y Antibody (PACO05595)

DDX3Y Antibody (PACO08792)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)