Anti-DDX41 Antibody (CAB6576)

Antibody Name:	Anti-DDX41 Antibody
Antibody SKU:	CAB6576
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human DDX41 (NP_057306.2).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, 293T, HL-60, MCF7, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human DDX41 (NP_057306.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEES EPER KRAR TDEV PAGG SRSE AEDE DDED YVPY VPLR QRRQ LLLQ KLLQ RRRK GAAE EEQQ DSGS EPRG DEDD IPLG PQSN VSLL DQHQ HLKE KAEA RKES AKEK QLKE EEKI LESV AEGR ALMS VKEM AKGI TYDD PIKT SWTP PRYV LSMS EERH ERVR KKYH ILVE GDGI PPPI KSFK EMKF PAAI LRGL KKKG IHHP TPIQ IQGI PTIL SGRD MIGI AFTG SGKT LVFT LPVI MFCL EQEK RLPF SKRE GPYG LIIC PSRE LARQ THGI LEYY CRLL QEDS SPLL RCAL CIGG
Gene ID:	51428
Uniprot:	Q9UJV9
Cellular Location:	Nucleus
Calculated MW:	69kDa
Observed MW:	75kDa

Synonyms:

DDX41, ABS, MPLPF

Background:

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a member of the DEAD box protein family and interacts with several spliceosomal proteins. In addition, the encoded protein may recognize the bacterial second messengers cyclic di-GMP and cyclic di-AMP, resulting in the induction of genes involved in the innate immune response.

UniProt Protein Function:	DDX41: Probable ATP-dependent RNA helicase. Is required during post-transcriptional gene expression. May be involved in pre-mRNA splicing. Belongs to the DEAD box helicase family. DDX41 subfamily.
UniProt Protein Details:	Protein type:Spliceosome; RNA-binding; RNA splicing; EC 3.6.4.13; Helicase Chromosomal Location of Human Ortholog: 5q35.3 Cellular Component: membrane; endoplasmic reticulum; cytosol Molecular Function:protein binding; DNA binding; zinc ion binding; helicase activity; ATP binding Biological Process: RNA processing; nuclear mRNA splicing, via spliceosome; regulation of interferon type I production; apoptosis; multicellular organismal development; positive regulation of interferon type I production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; defense response to virus
NCBI Summary:	DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The function of this member has not been determined. Based on studies in Drosophila, the abstrakt gene is widely required during post-transcriptional gene expression. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9UJV9
NCBI GenInfo Identifier:	20532370
NCBI Gene ID:	51428
NCBI Accession:	Q9UJV9.2
UniProt Secondary Accession:	Q9UJV9,Q96BK6, Q96K05, Q9NT96, Q9NW04, B2RDC8,
UniProt Related Accession:	Q9UJV9
Molecular Weight:	622
NCBI Full Name:	Probable ATP-dependent RNA helicase DDX41
NCBI Synonym Full Names:	DEAD (Asp-Glu-Ala-Asp) box polypeptide 41
NCBI Official Symbol:	DDX41
NCBI Official Synonym Symbols:	ABS
NCBI Protein Information:	probable ATP-dependent RNA helicase DDX41; 2900024F02Rik; DEAD box protein 41; putative RNA helicase; DEAD-box protein abstrakt; DEAD box protein abstrakt homolog
UniProt Protein Name:	Probable ATP-dependent RNA helicase DDX41
UniProt Synonym Protein Names:	DEAD box protein 41; DEAD box protein abstrakt homolog
Protein Family:	Probable ATP-dependent RNA helicase
UniProt Gene Name:	DDX41
UniProt Entry Name:	DDX41_HUMAN

	Western blot analysis of extracts of various cell lines, using DDX41 antibody (CAB6576) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat kidney using DDX41 antibody (CAB6576) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat testis using DDX41 antibody (CAB6576) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse testis using DDX41 antibody (CAB6576) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using DDX41 antibody (CAB6576). Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)