Anti-DNASE1 Antibody (CAB2537)

Antibody Name:	Anti-DNASE1 Antibody
Antibody SKU:	CAB2537
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 22-282 of human DNASE1 (NP_005214.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT-474, HT-29, Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 22-282 of human DNASE1 (NP_005214.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SLKI AAFN IQTF GETK MSNA TLVS YIVQ ILSR YDIA LVQE VRDS HLTA VGKL LDNL NQDA PDTY HYVV SEPL GRNS YKER YLFV YRPD QVSA VDSY YYDD GCEP CGND TFNR EPAI VRFF SRFT EVRE FAIV PLHA APGD AVAE IDAL YDVY LDVQ EKWG LEDV MLMG DFNA GCSY VRPS QWSS IRLW TSPT FQWL IPDS ADTT ATPT HCAY DRIV VAGM LLRG AVVP DSAL PFNF QAAY GLSD QLAQ AISD HYPV EVML K
Gene ID:	1773
Uniprot:	P24855
Cellular Location:	Nucleus envelope, Secreted
Calculated MW:	19kDa/31kDa
Observed MW:	37kDa

Synonyms:

DNASE1, DNL1, DRNI

Background:

This gene encodes a member of the DNase family. This protein is stored in the zymogen granules of the nuclear envelope and functions by cleaving DNA in an endonucleolytic manner. At least six autosomal codominant alleles have been characterized, DNASE1*1 through DNASE1*6, and the sequence of DNASE1*2 represented in this record. Mutations in this gene have been associated with systemic lupus erythematosus (SLE), an autoimmune disease. A recombinant form of this protein is used to treat the one of the symptoms of cystic fibrosis by hydrolyzing the extracellular DNA in sputum and reducing its viscosity. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

UniProt Protein Function:	DNASE1: Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization. Defects in DNASE1 are a cause of susceptibility to systemic lupus erythematosus (SLE). A chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Belongs to the DNase I family.
UniProt Protein Details:	Protein type:Deoxyribonuclease; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide; EC 3.1.21.1 Chromosomal Location of Human Ortholog: 16p13.3 Cellular Component: extracellular region; nuclear envelope; nucleus Molecular Function:protein binding; deoxyribonuclease I activity; actin binding; endodeoxyribonuclease activity Biological Process: apoptosis; DNA catabolic process, endonucleolytic Disease: Systemic Lupus Erythematosus
NCBI Summary:	This gene encodes a member of the DNase family. This protein is stored in the zymogen granules of the nuclear envelope and functions by cleaving DNA in an endonucleolytic manner. At least six autosomal codominant alleles have been characterized, DNASE11 through DNASE16, and the sequence of DNASE1*2 represented in this record. Mutations in this gene have been associated with systemic lupus erythematosus (SLE), an autoimmune disease. A recombinant form of this protein is used to treat the one of the symptoms of cystic fibrosis by hydrolyzing the extracellular DNA in sputum and reducing its viscosity. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008]
UniProt Code:	P24855
NCBI GenInfo Identifier:	118919
NCBI Gene ID:	1773
NCBI Accession:	P24855.1
UniProt Secondary Accession:	P24855,Q14UU9, Q14UV0, B4DV35,
UniProt Related Accession:	P24855
Molecular Weight:	Calculated: 19kDa/31kDaObserved: 37kDa
NCBI Full Name:	Deoxyribonuclease-1
NCBI Synonym Full Names:	deoxyribonuclease I
NCBI Official Symbol:	DNASE1
NCBI Official Synonym Symbols:	DNL1; DRNI
NCBI Protein Information:	deoxyribonuclease-1; Dornase alfa; DNase I, lysosomal; human urine deoxyribonuclease I
UniProt Protein Name:	Deoxyribonuclease-1
UniProt Synonym Protein Names:	Deoxyribonuclease I; DNase I; INN: Dornase alfa
Protein Family:	Deoxyribonuclease
UniProt Gene Name:	DNASE1
UniProt Entry Name:	DNAS1_HUMAN

	Western blot analysis of extracts of various cell lines, using DNASE1 antibody (CAB2537) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of HeLa cells using DNASE1 antibody (CAB2537).

Antibodies	ELISA Kits
Anti-DNASE1 Antibody (CAB14531)	Human Deoxyribonuclease-1 / DNASE1 ELISA Kit
Anti-DNASE1 Antibody (CAB17473)	Human Deoxyribonuclease-1 (DNASE1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)