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Anti-DGKA Antibody (CAB13969)

SKU:
CAB13969
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
€139 - €419
Frequently bought together:

Description

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Antibody Name:Anti-DGKA Antibody
Antibody SKU:CAB13969
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human DGKA (NP_958852.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:NCI-H460, MCF7, HeLa, Mouse lung, Rat lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human DGKA (NP_958852.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAKE RGLI SPSD FAQL QKYM EYST KKVS DVLK LFED GEMA KYVQ GDAI GYEG FQQF LKIY LEVD NVPR HLSL ALFQ SFET GHCL NETN VTKD VVCL NDVS CYFS LLEG GRPE DKLE FTFK LYDT DRNG ILDS SEVD KIIL QMMR VAEY LDWD VSEL RPIL QEMM KEID YDGS GSVS QAEW VRAG ATTV PLLV LLGL EMTL
Gene ID:1606
Uniprot:P23743
Cellular Location:
Calculated MW:13kDa/28kDa/82kDa
Observed MW:83kDa
Synonyms:DGKA, DAGK, DAGK1, DGK-alpha
Background:The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Several transcript variants encoding different isoforms have been identified for this gene.
UniProt Protein Function:DGKA: Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity. Monomer. Lymphocytes and oligodendroglial cells. Stimulated by calcium and phosphatidylserine. Phosphorylated by protein kinase C. Belongs to the eukaryotic diacylglycerol kinase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Lipid Metabolism - glycerophospholipid; EC 2.7.1.107; Motility/polarity/chemotaxis; Lipid Metabolism - glycerolipid; Kinase, lipid

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: membrane; plasma membrane; cytosol

Molecular Function:phospholipid binding; calcium ion binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity

Biological Process: platelet activation; protein kinase C activation; blood coagulation; phosphorylation

NCBI Summary:The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:P23743
NCBI GenInfo Identifier:281185505
NCBI Gene ID:1606
NCBI Accession:P23743.3
UniProt Secondary Accession:P23743,O75481, O75482, O75483, O95217, Q3ZE25, Q8IZ56 Q8N5Q2,
UniProt Related Accession:P23743
Molecular Weight:Calculated: 13kDa/28kDa/82kDaObserved: 83kDa
NCBI Full Name:Diacylglycerol kinase alpha
NCBI Synonym Full Names:diacylglycerol kinase, alpha 80kDa
NCBI Official Symbol:DGKA  
NCBI Official Synonym Symbols:DAGK; DAGK1; DGK-alpha  
NCBI Protein Information:diacylglycerol kinase alpha; DAG kinase alpha; diglyceride kinase alpha; 80 kDa diacylglycerol kinase
UniProt Protein Name:Diacylglycerol kinase alpha
UniProt Synonym Protein Names:80 kDa diacylglycerol kinase; Diglyceride kinase alpha; DGK-alpha
Protein Family:Diacylglycerol kinase
UniProt Gene Name:DGKA  
UniProt Entry Name:DGKA_HUMAN
Anti-DGKA Antibody (CAB13969)Western blot analysis of extracts of various cell lines, using DGKA antibody (CAB13969) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.

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