Anti-DHX38 Antibody (CAB4341)

Antibody Name:	Anti-DHX38 Antibody
Antibody SKU:	CAB4341
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1133-1227 of human DHX38 (NP_054722.2).

Application:	WB IHC
Recommended Dilution:	WB 1:1000 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, SKOV3, U-87MG, MCF7, DU145, Mouse testis, Mouse thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1133-1227 of human DHX38 (NP_054722.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VTAV DGEW LAEL GPMF YSVK QAGK SRQE NRRR AKEE ASAM EEEM ALAE EQLR ARRQ EQEK RSPL GSVR STKI YTPG RKEQ GEPM TPRR TPAR FGL
Gene ID:	9785
Uniprot:	Q92620
Cellular Location:	Nucleus
Calculated MW:	60kDa/140kDa
Observed MW:	140kDa

Synonyms:

DHX38, DDX38, PRP16, PRPF16

Background:

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a member of the DEAD/H box family of splicing factors. This protein resembles yeast Prp16 more closely than other DEAD/H family members. It is an ATPase and essential for the catalytic step II in pre-mRNA splicing process.

UniProt Protein Function:	DHX38: Probable ATP-binding RNA helicase involved in pre-mRNA splicing. Belongs to the DEAD box helicase family. DEAH subfamily. PRP16 sub-subfamily.
UniProt Protein Details:	Protein type:Spliceosome; EC 3.6.4.13; RNA-binding; RNA processing; Helicase; RNA splicing Chromosomal Location of Human Ortholog: 16q22 Cellular Component: nucleoplasm; membrane; cytoplasm; nucleus Molecular Function:protein binding; ATP-dependent RNA helicase activity; ATP binding Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; mRNA export from nucleus; RNA splicing; gene expression; mRNA 3'-end processing; termination of RNA polymerase II transcription
NCBI Summary:	DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a member of the DEAD/H box family of splicing factors. This protein resembles yeast Prp16 more closely than other DEAD/H family members. It is an ATPase and essential for the catalytic step II in pre-mRNA splicing process. [provided by RefSeq, Jul 2008]
UniProt Code:	Q92620
NCBI GenInfo Identifier:	85700389
NCBI Gene ID:	9785
NCBI Accession:	Q92620.2
UniProt Related Accession:	Q92620
Molecular Weight:	140kDa
NCBI Full Name:	Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16
NCBI Synonym Full Names:	DEAH-box helicase 38
NCBI Official Symbol:	DHX38
NCBI Official Synonym Symbols:	RP84; DDX38; PRP16; PRPF16
NCBI Protein Information:	pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16
UniProt Protein Name:	Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16
UniProt Synonym Protein Names:	ATP-dependent RNA helicase DHX38; DEAH box protein 38
UniProt Gene Name:	DHX38
UniProt Entry Name:	PRP16_HUMAN

	Western blot analysis of extracts of various cell lines, using DHX38 antibody (CAB4341) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s.
	Immunohistochemistry of paraffin-embedded human gastric cancer using DHX38 antibody (CAB4341) at dilution of 1:150 (40x lens).

Antibodies

DHX38 Antibody (PACO08834)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)