Anti-DIABLO Antibody (CAB2564)

Antibody Name:	Anti-DIABLO Antibody
Antibody SKU:	CAB2564
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-232 of human DIABLO (NP_063940.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW620, BT-474, Mouse spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-232 of human DIABLO (NP_063940.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAL KSWL SRSV TSFF RYRQ CLCV PVVA NFKK RCFS ELIR PWHK TVTI GFGV TLCA VPIA QKSE PHSL SSEA LMRR AVSL VTDS TSTF LSQT TYAL IEAI TEYT KAVY TLTS LYRQ YTSL LGKM NSEE EDEV WQVI IGAR AEMT SKHQ EYLK LETT WMTA VGLS EMAA EAAY QTGA DQAS ITAR NHIQ LVKL QVEE VHQL SRKA ETKL AEAQ IEEL RQKT QEEG EERA ESEQ
Gene ID:	56616
Uniprot:	Q9NR28
Cellular Location:	Mitochondrion
Calculated MW:	21kDa/22kDa/27kDa
Observed MW:	18kDa

Synonyms:

DIABLO, DFNA64, SMAC

Background:

This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64. Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	DIABLO: Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis- inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance. Homodimer. Interacts with NGFRAP1/BEX3. Interacts with BIRC2/c-IAP1, BIRC3/c-IAP2, XIAP/BIRC4, BIRC6/bruce and BIRC7/livin. Interacts with the monomeric and dimeric form of BIRC5/survivin. Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Mitochondrial; Apoptosis Chromosomal Location of Human Ortholog: 12q24.31 Cellular Component: internal side of plasma membrane; mitochondrion; mitochondrial intermembrane space; cytosol Molecular Function:protein binding Biological Process: caspase activation; neuron apoptosis; induction of apoptosis via death domain receptors; apoptosis; positive regulation of apoptosis; caspase activation via cytochrome c; induction of apoptosis by oxidative stress Disease: Deafness, Autosomal Dominant 64
NCBI Summary:	This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2013]
UniProt Code:	Q9NR28
NCBI GenInfo Identifier:	18203316
NCBI Gene ID:	56616
NCBI Accession:	Q9NR28.1
UniProt Secondary Accession:	Q9NR28,Q6W3F3, Q96LV0, Q9BT11, Q9HAV6, B2RDQ0,
UniProt Related Accession:	Q9NR28
Molecular Weight:	239
NCBI Full Name:	Diablo homolog, mitochondrial
NCBI Synonym Full Names:	diablo, IAP-binding mitochondrial protein
NCBI Official Symbol:	DIABLO
NCBI Official Synonym Symbols:	SMAC; DFNA64
NCBI Protein Information:	diablo homolog, mitochondrial; direct IAP-binding protein with low pI; second mitochondria-derived activator of caspase
UniProt Protein Name:	Diablo homolog, mitochondrial
UniProt Synonym Protein Names:	Direct IAP-binding protein with low pI; Second mitochondria-derived activator of caspase; Smac
Protein Family:	Diablo
UniProt Gene Name:	DIABLO
UniProt Entry Name:	DBLOH_HUMAN

	Western blot analysis of extracts of various cell lines, using DIABLO antibody (CAB2564) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded rat testis using DIABLO Rabbit pAb (CAB2564) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse testis using DIABLO Rabbit pAb (CAB2564) at dilution of 1:100 (40x lens).

Antibodies

Anti-DIABLO Antibody (CAB13432)

Anti-Smac / Diablo Antibody (CAB8889)

DIABLO Antibody (PACO08837)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)