Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dicer Monoclonal Antibody, Clone N167/7 (STMA00126). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Dicer Monoclonal Antibody (STMA00126) at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT.
Key Features
Anti-Dicer Antibody [N167/7 (Formerly sold as S167-7)]
Dicer Antibody [N167/7 (Formerly sold as S167-7)] is a mouse monoclonal antibody directed against Dicer, offered by Assay Genie for research applications. Reported applications include WB and ICC/IF. Reported reactivity: Human, Mouse and Rat. Supplied as IgG1, purified by Protein G.
Dicer is a ribonuclease III enzyme essential for the biogenesis of microRNAs (miRNAs), which regulate gene expression post-transcriptionally. It processes precursor miRNAs into mature ~22-nucleotide miRNAs that guide the RNA-induced silencing complex (RISC) to target mRNAs. In the nervous system, Dicer is critical for neuronal differentiation, synaptic plasticity, and stress responses. Dysregulation of Dicer or miRNA pathways has been implicated in neurodegenerative diseases, including Alzheimer’s, ALS, and Huntington’s disease. Loss of Dicer function leads to widespread transcriptomic imbalance and neuronal vulnerability, making it a key player in epigenetic regulation and a potential therapeutic target in neurodegeneration. This antibody is also available conjugated to ATTO 390, ATTO 488, ATTO 594, APC, Biotin, FITC, HRP, PerCP and RPE. Supplied in 100 µg. For research use only; not for diagnostic or therapeutic procedures.
Product Name:
Dicer Antibody [N167/7 (Formerly sold as S167-7)]
Product SKU:
STMA00126
Size:
100 µg
Target:
Dicer
Host Species:
Mouse
Antibody Type:
Monoclonal
Clone ID:
N167/7 (Formerly sold as S167-7)
Research Areas:
Cell Signaling, Epigenetics and Nuclear Signaling
Buffer:
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Storage & Shipping:
Store at -20°C. Shipped on Blue Ice or 4°C. Conjugated variants should be stored according to the product label.
Format:
Liquid
Applications:
WB, ICC/IF
Antibody Isotype:
IgG1
Purification:
Protein G Purified
Concentration:
1 mg/ml
Reactivity:
Human, Mouse, Rat
Recommended Dilution:
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Specificity:
Detects ~215kDa.
Guarantee:
12 months from date of dispatch
Immunogen:
Fusion protein amino acids 1638-1899 of mouse Endoribonuclease Dicer
1. Hutvágner G. and Zamore P.D. (2002) Science 297: 2056-60. 2. Lee Y., et al. (2003) Nature 425: 415-9. 3. Diederichs S. and Haber, D.A. (2007) Cell 131: 1097-108.
