Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-LGI1 Monoclonal Antibody, Clone N283/7 (STMA00154). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-LGI1 Monoclonal Antibody (STMA00154) at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT.
Key Features
Anti-LGI1 Antibody [N283/7 (Formerly sold as S283-7)]
LGI1 Antibody [N283/7 (Formerly sold as S283-7)] is a mouse monoclonal antibody directed against LGI1, offered by Assay Genie for research applications. Reported applications include WB, IHC and ICC/IF. Reported reactivity: Human, Mouse and Rat. Supplied as IgG2a, purified by Protein G.
LGI1 (Leucine-Rich Glioma Inactivated 1) is a secreted synaptic protein that modulates neuronal excitability and synaptic transmission. It interacts with ADAM22 and ADAM23 to stabilize AMPA receptor function and maintain synaptic integrity. Mutations in LGI1 are linked to autosomal dominant lateral temporal lobe epilepsy (ADTLE) and have been implicated in autoimmune encephalitis. In neurodegenerative disease research, LGI1 is gaining attention for its role in synaptic maintenance, neuroinflammation, and neuronal survival. Dysregulation of LGI1 may contribute to cognitive decline and seizure susceptibility. Its dual role in epilepsy and neurodegeneration highlights LGI1 as a valuable biomarker and therapeutic target in synaptic pathologies. This antibody is also available conjugated to ATTO 390, ATTO 488, ATTO 594, APC, Biotin, FITC, HRP, PerCP and RPE. Supplied in 100 µg. For research use only; not for diagnostic or therapeutic procedures.
Product Name:
LGI1 Antibody [N283/7 (Formerly sold as S283-7)]
Product SKU:
STMA00154
Size:
100 µg
Target:
LGI1
Host Species:
Mouse
Antibody Type:
Monoclonal
Clone ID:
N283/7 (Formerly sold as S283-7)
Research Areas:
Neuroscience, Neurodegeneration, Cell Signaling, Epigenetics and Nuclear Signaling, Cancer
Buffer:
PBS pH7.4, 50% glycerol, 0.1% sodium azide
Storage & Shipping:
Store at -20°C. Shipped on Blue Ice or 4°C. Conjugated variants should be stored according to the product label.
Format:
Liquid
Applications:
WB, IHC, ICC/IF
Antibody Isotype:
IgG2a
Purification:
Protein G Purified
Concentration:
1 mg/ml
Reactivity:
Human, Mouse, Rat
Recommended Dilution:
WB (1:1000); optimal dilutions for assays should be determined by the user.
Specificity:
Detects ~60kDa.
Guarantee:
12 months from date of dispatch
Immunogen:
Fusion protein amino acids 37-113 (LRRNT domain and first LRR repeat) of mouse LGI1. Rat: 100% identity (77/77 amino acids identical). Human: 98% identity (76/77 amino acids identical). ~50% identity with LGI2, LGI3 and LGI4.
