Anti-DLC1 Antibody (CAB1921)

Antibody Name:	Anti-DLC1 Antibody
Antibody SKU:	CAB1921
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 204-463 of human DLC1 (NP_079043.3).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Mouse, Rat
Positive Samples:	Mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 204-463 of human DLC1 (NP_079043.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DAPK VNAV DTLN VKDI APEK QLLN SAVI AQQR RKPD PPKD ENER STCN VVQN EFLD TPCT NRGL PLLK TDFG SCLL QPPS CPNG MSAE NGLE KSGF SQHQ NKSP PKVK AEDG MQCL QLKE TLAT QEPT DNQV RLRK RKEI REDR DRAR LDSM VLLI MKLD QLDQ DIEN ALST SSSP SGTP TNLR RHVP DLES GSES GADT ISVN QTRV NLSS DTES TDLP SSTP VANS GTKP KTTA IQGI SEKE KAGK LTFW FCFL ANLF
Gene ID:	10395
Uniprot:	Q96QB1
Cellular Location:	Cell junction, Cytoplasm, Membrane, Peripheral membrane protein, focal adhesion
Calculated MW:	50kDa/54kDa/114kDa/122kDa/126kDa/170kDa
Observed MW:	171kDa

Synonyms:

DLC1, ARHGAP7, HP, STARD12, p122-RhoGAP

Background:

This gene encodes a GTPase-activating protein (GAP) that is a member of the rhoGAP family of proteins which play a role in the regulation of small GTP-binding proteins. GAP family proteins participate in signaling pathways that regulate cell processes involved in cytoskeletal changes. This gene functions as a tumor suppressor gene in a number of common cancers, including prostate, lung, colorectal, and breast cancers. Multiple transcript variants due to alternative promoters and alternative splicing have been found for this gene.

UniProt Protein Function:	DLC1: Functions as a GTPase-activating protein specific for Rho and an activator of PLCD1 in vivo and induces morphological changes and detachment through cytoskeletal reorganization. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:GAPs; GAPs, Rac/Rho; Tumor suppressor Chromosomal Location of Human Ortholog: 8p22 Cellular Component: cortical actin cytoskeleton; focal adhesion; cytoplasm; caveola; cytosol; nucleus Molecular Function:protein binding; SH2 domain binding; lipid binding Biological Process: caspase activation; focal adhesion formation; heart morphogenesis; hindbrain morphogenesis; negative regulation of stress fiber formation; apoptosis; negative regulation of focal adhesion formation; positive regulation of protein amino acid dephosphorylation; regulation of cell shape; negative regulation of cell proliferation; regulation of small GTPase mediated signal transduction; negative regulation of Rho protein signal transduction; small GTPase mediated signal transduction; regulation of actin cytoskeleton organization and biogenesis; neural tube closure; forebrain development; actin cytoskeleton organization and biogenesis; negative regulation of cell migration Disease: Colorectal Cancer
NCBI Summary:	This gene encodes a GTPase-activating protein (GAP) that is a member of the rhoGAP family of proteins which play a role in the regulation of small GTP-binding proteins. GAP family proteins participate in signaling pathways that regulate cell processes involved in cytoskeletal changes. This gene functions as a tumor suppressor gene in a number of common cancers, including prostate, lung, colorectal, and breast cancers. Multiple transcript variants due to alternative promoters and alternative splicing have been found for this gene.[provided by RefSeq, Apr 2010]
UniProt Code:	Q96QB1
NCBI GenInfo Identifier:	313104315
NCBI Gene ID:	10395
NCBI Accession:	Q96QB1.4
UniProt Secondary Accession:	Q96QB1,O14868, O43199, Q7Z5R8, Q86UC6, Q9C0E0, Q9H7A2 B4DR10, B8PTI0, E9PDZ8, E9PF76, E9PGY9,
UniProt Related Accession:	Q96QB1
Molecular Weight:	1528
NCBI Full Name:	Rho GTPase-activating protein 7
NCBI Synonym Full Names:	DLC1 Rho GTPase activating protein
NCBI Official Symbol:	DLC1
NCBI Official Synonym Symbols:	HP; ARHGAP7; STARD12; p122-RhoGAP
NCBI Protein Information:	rho GTPase-activating protein 7; Rho-GTPase-activating protein 7; deleted in liver cancer 1 protein; START domain-containing protein 12; deleted in liver cancer 1 variant 2; rho-type GTPase-activating protein 7; StAR-related lipid transfer (START) domain containing 12
UniProt Protein Name:	Rho GTPase-activating protein 7
UniProt Synonym Protein Names:	Deleted in liver cancer 1 protein; DLC-1; HP protein; Rho-type GTPase-activating protein 7; START domain-containing protein 12; StARD12; StAR-related lipid transfer protein 12
Protein Family:	Dynein
UniProt Gene Name:	DLC1
UniProt Entry Name:	RHG07_HUMAN

	Western blot analysis of extracts of mouse lung, using DLC1 antibody (CAB1921) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021).
	Immunohistochemistry of paraffin-embedded rat kidney using DLC1 antibody (CAB1921) at dilution of 1:200 (40x lens).

Antibodies

DLC1 Antibody (PACO03340)

DLC1 Antibody (PACO08849)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)