DNA Polymerase beta Rabbit Monoclonal Antibody (CAB2412)

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CAB2412
€129 - €349

Description

Technical Manual

DNA Polymerase beta Rabbit Monoclonal Antibody

Product Name:DNA Polymerase beta Rabbit Monoclonal Antibody
Product SKU:CAB2412
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. [provided by RefSeq, Sep 2011]

Immunogen:A synthesized peptide derived from human DNA Polymerase beta
Sequence:

Email for sequence

Gene ID:5423
Swiss Prot:P06746
Synonyms:
Calculated MW:38kDa
Observed MW:42KDa
Reactivity:Human, Mouse, Rat
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application Key:Western blotting Immunohistochemistry 
Positive Samples:HeLa, A-431, Jurkat, Mouse testis, Mouse spleen, Mouse thymus, Rat testis, Rat spleen
Cellular Location:

Product Images

DNA Polymerase beta Rabbit Monoclonal Antibody (CAB2412)

Western blot - DNA Polymerase beta Rabbit mAb (CAB2412)

Uniprot Information

UniProt Protein Function:POLB: Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Monomer. Interacts with APEX1, HUWE1/ARF-BP1, STUB1/CHIP and USP47. Belongs to the DNA polymerase type-X family.
UniProt Protein Details:

Protein type:EC 2.7.7.7; Transferase; Lyase; DNA replication; DNA repair, damage

Chromosomal Location of Human Ortholog: 8p11.2

Cellular Component: nucleoplasm; microtubule; spindle microtubule; cytoplasm; nucleus

Molecular Function:protein binding; enzyme binding; metal ion binding; microtubule binding; damaged DNA binding; DNA-directed DNA polymerase activity; lyase activity

Biological Process: base-excision repair, gap-filling; neuron apoptosis; response to ethanol; DNA damage response, signal transduction resulting in induction of apoptosis; base-excision repair; pyrimidine dimer repair; nucleotide-excision repair, DNA gap filling; DNA repair; DNA-dependent DNA replication; response to DNA damage stimulus

NCBI Summary:The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. [provided by RefSeq, Sep 2011]
UniProt Code:P06746
NCBI GenInfo Identifier:4505931
NCBI Gene ID:5423
NCBI Accession:NP_002681.1
UniProt Related Accession:P06746
Molecular Weight:~ 38kDa
NCBI Full Name:DNA polymerase beta
NCBI Synonym Full Names:DNA polymerase beta
NCBI Official Symbol:POLB 
NCBI Protein Information:DNA polymerase beta
UniProt Protein Name:DNA polymerase beta
UniProt Gene Name:POLB 
UniProt Entry Name:DPOLB_HUMAN
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Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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