The DNA polymerase eta Antibody (CAB1833) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, specifically targets DNA polymerase Eta, a specialized enzyme involved in translesion synthesis that allows for the bypass of DNA lesions during replication. With high reactivity towards human samples, this antibody is perfect for use in Western blot applications, enabling the detection and analysis of DNA polymerase Eta in various cell types. Its specificity makes it an ideal choice for research in fields such as genetics, molecular biology, and cancer biology, where understanding DNA repair mechanisms is crucial.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
DNA polymerase eta Antibody
SKU:
CAB1833
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
XPV, XP-V, RAD30, RAD30A, DNA polymerase eta
Positive Sample:
Jurkat, HepG2, Mouse thymus, Mouse spleen
Cellular Localization:
Nucleus.
Calculated MW:
78kDa
Observed MW:
80kDa
This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA; when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
5429
RRID
AB_2763870
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using DNA polymerase eta Rabbit pAb (CAB1833) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunofluorescence analysis of U-2 OS cells using DNA polymerase eta Rabbit pAb (CAB1833) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
