Anti-DNAJB6 Antibody (CAB7052)

Antibody Name:	Anti-DNAJB6 Antibody
Antibody SKU:	CAB7052
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 177-326 of human DNAJB6 (NP_490647.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW480, MCF7, 293T, Jurkat, HeLa, Mouse testis, Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 177-326 of human DNAJB6 (NP_490647.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	STSF GGSG MGNF KSIS TSTK MVNG RKIT TKRI VENG QERV EVEE DGQL KSLT INGV ADDD ALAE ERMR RGQN ALPA QPAG LRPP KPPR PASL LRHA PHCL SEEE GEQD RPRA PGPW DPLA SAAG LKEG GKRK KQKQ REES KKKK STKG NH
Gene ID:	10049
Uniprot:	O75190
Cellular Location:	Cytoplasm, Nucleus, Z line, myofibril, perinuclear region, sarcomere
Calculated MW:	26kDa/30kDa/35kDa/36kDa
Observed MW:	40kDa

Synonyms:

DNAJB6, DJ4, DnaJ, HHDJ1, HSJ-2, HSJ2, LGMD1D, LGMD1E, MRJ, MSJ-1

Background:

This gene encodes a member of the DNAJ protein family. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. This family member may also play a role in polyglutamine aggregation in specific neurons. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been fully described.

UniProt Protein Function:	DNAJB6: Plays an indispensable role in the organization of KRT8/KRT18 filaments. Acts as an endogenous molecular chaperone for neuronal proteins including huntingtin. Suppresses aggregation and toxicity of polyglutamine-containing, aggregation-prone proteins. Isoform B but not isoform A inhibits huntingtin aggregation. Has a stimulatory effect on the ATPase activity of HSP70 in a dose-dependent and time-dependent manner and hence acts as a co-chaperone of HSP70. Also reduces cellular toxicity and caspase-3 activity. Defects in DNAJB6 are the cause of limb-girdle muscular dystrophy type 1E (LGMD1E). An autosomal dominant myopathy characterized by adult onset of proximal muscle weakness, beginning in the hip girdle region and later progressing to the shoulder girdle region. There is evidence that LGMD1E is caused by dysfunction of isoform B (PubMed:22366786). 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Chaperone Chromosomal Location of Human Ortholog: 7q36.3 Cellular Component: cytosol; membrane; nucleoplasm; nucleus; perinuclear region of cytoplasm; Z disc Molecular Function:ATPase activator activity; chaperone binding; heat shock protein binding; protein binding; unfolded protein binding Biological Process: intermediate filament organization; negative regulation of caspase activity; protein folding; regulation of protein localization Disease: Muscular Dystrophy, Limb-girdle, Type 1e
NCBI Summary:	This gene encodes a member of the DNAJ protein family. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. This family member may also play a role in polyglutamine aggregation in specific neurons. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]
UniProt Code:	O75190
NCBI GenInfo Identifier:	19855067
NCBI Gene ID:	10049
NCBI Accession:	O75190.2
UniProt Secondary Accession:	O75190,O95806, Q53EN8, Q59EF2, Q6FIC8, Q75MA2, Q9UIK6 A4D232, A8K7D8, A8KAG0, B4DN73, E9PCZ2,
UniProt Related Accession:	O75190
Molecular Weight:	30,369 Da
NCBI Full Name:	DnaJ homolog subfamily B member 6
NCBI Synonym Full Names:	DnaJ heat shock protein family (Hsp40) member B6
NCBI Official Symbol:	DNAJB6
NCBI Official Synonym Symbols:	DJ4; MRJ; DnaJ; HSJ2; HHDJ1; HSJ-2; MSJ-1; LGMD1D; LGMD1E
NCBI Protein Information:	dnaJ homolog subfamily B member 6
UniProt Protein Name:	DnaJ homolog subfamily B member 6
UniProt Synonym Protein Names:	HHDJ1; Heat shock protein J2; HSJ-2; MRJ; MSJ-1
Protein Family:	DnaJ homolog subfamily
UniProt Gene Name:	DNAJB6
UniProt Entry Name:	DNJB6_HUMAN

	Western blot analysis of extracts of various cell lines, using DNAJB6 antibody (CAB7052) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human kidney cancer using DNAJB6 antibody (CAB7052) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse ileum using DNAJB6 antibody (CAB7052) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using DNAJB6 antibody (CAB7052). Blue: DAPI for nuclear staining.

Antibodies

DNAJB6 Antibody (PACO08882)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)