Anti-DNAL1 Antibody (CAB8267)

Antibody Name:	DNAL1 Rabbit Polyclonal Antibody
Antibody SKU:	CAB8267
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 41-190 of human DNAL1 (NP_113615.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 41-190 of human DNAL1 (NP_113615.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DASL SMLA NCEK LSLS TNCI EKIA NLNG LKNL RILS LGRN NIKN LNGL EAVG DTLE ELWI SYNF IEKL KGIH IMKK LKIL YMSN NLVK DWAE FVKL AELP CLED LVFV GNPL EEKH SAEN NWIE EATK RVPK LKKL DGTP VIKG DEEE DN
Gene ID:	83544
Uniprot:	Q4LDG9
Cellular Location:
Calculated MW:	8kDa/17kDa/21kDa
Observed MW:	22kDa

Synonyms:

DNAL1, C14orf168, CILD16

Background:

This gene encodes an axonemal dynein light chain which functions as a component of the outer dynein arms complex. This complex acts as the molecular motor that provides the force to move cilia in an ATP-dependent manner. The encoded protein is expressed in tissues with motile cilia or flagella and may be involved in the movement of sperm flagella. Alternate splicing results in multiple transcript variants.

UniProt Protein Function:	DNAL1: Defects in DNAL1 are the cause of primary ciliary dyskinesia type 16 (CILD16). A disorder characterized by abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia; reduced fertility is often observed in male patients due to abnormalities of sperm tails. Half of the patients exhibit randomization of left-right body asymmetry and situs inversus, due to dysfunction of monocilia at the embryonic node. Primary ciliary dyskinesia associated with situs inversus is referred to as Kartagener syndrome. Belongs to the dynein light chain LC1-type family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 14q24.3 Disease: Ciliary Dyskinesia, Primary, 16
NCBI Summary:	This gene encodes an axonemal dynein light chain which functions as a component of the outer dynein arms complex. This complex acts as the molecular motor that provides the force to move cilia in an ATP-dependent manner. The encoded protein is expressed in tissues with motile cilia or flagella and may be involved in the movement of sperm flagella. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Jan 2011]
UniProt Code:	Q4LDG9
NCBI GenInfo Identifier:	121944344
NCBI Gene ID:	83544
NCBI Accession:	Q4LDG9.1
UniProt Secondary Accession:	Q4LDG9,Q5JPB7, Q9BS43, B2RD38,
UniProt Related Accession:	Q4LDG9
Molecular Weight:	22kDa
NCBI Full Name:	Dynein light chain 1, axonemal
NCBI Synonym Full Names:	dynein axonemal light chain 1
NCBI Official Symbol:	DNAL1
NCBI Official Synonym Symbols:	CILD16; C14orf168
NCBI Protein Information:	dynein light chain 1, axonemal
UniProt Protein Name:	Dynein light chain 1, axonemal
Protein Family:	Dynein
UniProt Gene Name:	DNAL1

	Western blot analysis of extracts of mouse testis, using DNAL1 antibody (CAB8267) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human stomach using DNAL1 antibody (CAB8267) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat kidney using DNAL1 antibody (CAB8267) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using DNAL1 antibody (CAB8267) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using DNAL1 antibody (CAB8267) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Anti-DNAL1 Antibody (CAB12601)

DNAL1 Antibody (PACO00729)

DNAL1 Antibody (PACO08895)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)