DNMT1 Antibody [60B1220.1] is a mouse monoclonal antibody directed against DNMT1, offered by Assay Genie for research applications. Reported applications include WB, IHC, IP and ChIP. Reported reactivity: Human, Mouse, Fish and Zebrafish (Danio rerio). Supplied as IgG1 Kappa, purified by Protein G.
DNMT1 (DNA methyltransferase 1) is the primary enzyme responsible for maintaining DNA methylation patterns during cell division. In the nervous system, DNMT1 regulates gene expression, synaptic plasticity, and neuronal differentiation through epigenetic mechanisms. Aberrant DNMT1 activity has been linked to neurodegenerative diseases, where altered methylation contributes to transcriptional silencing of neuroprotective genes and activation of pathogenic pathways. DNMT1 interacts with key regulatory proteins such as retinoblastoma (Rb), E2F1, and HDAC1, forming complexes that influence chromatin structure and gene repression. Loss of DNMT1 function leads to global hypomethylation, genomic instability, and impaired neuronal survival. Its role in epigenetic remodeling makes DNMT1 a promising target for therapeutic intervention and biomarker discovery in neurodegeneration. This antibody is also available conjugated to ATTO 390, ATTO 488, ATTO 594, APC, Biotin, FITC, HRP, PerCP and RPE. Supplied in 100 µg. For research use only; not for diagnostic or therapeutic procedures.
Store at -20°C. Shipped on Blue Ice or 4°C. Conjugated variants should be stored according to the product label.
Format:
Liquid
Applications:
WB, IHC, IP, ChIP
Antibody Isotype:
IgG1 Kappa
Purification:
Protein G Purified
Concentration:
1 mg/ml
Reactivity:
Human, Mouse, Fish, Zebrafish (Danio rerio)
Recommended Dilution:
WB (1:1000), IHC (1:1000); optimal dilutions for assays should be determined by the user.
Specificity:
Detects ~180kDa. It will cross-react with mouse DNMT1.
Guarantee:
12 months from date of dispatch
Immunogen:
Raised against a synthetic peptide corresponding to amino acids 637-650 of human DNMT1
Immunogen Species:
Human
Accession Number:
NP_001370
Gene ID:
1786
Swiss-Prot:
P26358
Cellular Localization:
Nucleus
Alternative Names:
DNA Mtase, DNMT, MCMT, DNA methyltransferase 1, AIM, CXXC9, DNA (cytosine-5)-methyltransferase 1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI
References:
1. Bestor T., et al.(1988) J.Mol. Biol. 203: 971-983. 2. Yen R.W., Vertino P.M., Nelkin B.D., et al. (1992) Nucl. Acids Res. 20: 2287-2291. 3. Xie S., et al. (1999) Gene 236: 87-95. 4. Okano M., Bell D.W., Haber D.A. and Li E. (1999) Cell 99: 247-257. 5. Reik W. et al. (1999) J. Nat. Genet 23: 380-382. 6. Robertson K.D., et al. (2000) Nat Genet 25(3): 338-342.
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