Anti-DNMT1 Antibody (CAB5495)

Antibody Name:	Anti-DNMT1 Antibody [KO Validated]
Antibody SKU:	CAB5495
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF IP
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human DNMT1 (NP_001370.1).

Application:	WB IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:	Human
Positive Samples:	293T, SW480

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human DNMT1 (NP_001370.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MPAR TAPA RVPT LAVP AISL PDDV RRRL KDLE RDSL TEKE CVKE KLNL LHEF LQTE IKNQ LCDL ETKL RKEE LSEE GYLA KVKS LLNK DLSL ENGA HAYN REVN GRLE NGNQ ARSE ARRV GMAD ANSP PKPL SKPR TPRR SKSD GEAK PEPS PSPR ITRK STRQ TTIT SHFA KGPA KRKP QEES ERAK SDES IKEE DKDQ DEKR RRVT SRER VARP LPAE EPER AKSG TRTE KEEE RDEK EEKR LRSQ TKEP TPKQ KLKE EPDR EARA GV
Gene ID:	1786
Uniprot:	P26358
Cellular Location:	Nucleus
Calculated MW:	144kDa/183kDa/184kDa
Observed MW:	200kDa

Synonyms:

ADCADN, AIM, CXXC9, DNMT, HSN1E, MCMT, m.HsaI, DNMT1

Background:

This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	DNMT1: an ubiquitous DNA methyltransferase that methylates CpG residues. Preferentially methylates hemimethylated DNA. It is responsible for maintaining methylation patterns established in development, and may play an active role in DNA damage repair. Mediates transcriptional repression by direct binding to HDAC2. Its abundance is reduced to non detectable levels at the G0 phase of the cell cycle and is dramatically induced upon entrance into the S-phase of the cell cycle. Interacts with HDAC1 and with PCNA. Forms a complex with DMAP1 and HDAC2, with direct interaction. Forms also a stable complex with E2F1, BB1 and HDAC1. Binds MBD2 and MBD3. Three isoforms of the human protein produced by alternative splicing have been described.
UniProt Protein Details:	Protein type:Methyltransferase, DNA; EC 2.1.1.37; Cell development/differentiation; Methyltransferase; Transcription regulation; Amino Acid Metabolism - cysteine and methionine Chromosomal Location of Human Ortholog: 19p13.2 Cellular Component: nucleoplasm; centric heterochromatin; replication fork; nucleus Molecular Function:DNA (cytosine-5-)-methyltransferase activity; protein binding; methyl-CpG binding; DNA binding; zinc ion binding; RNA binding; chromatin binding; DNA-methyltransferase activity; transcription factor binding Biological Process: negative regulation of histone H3-K9 methylation; transcription, DNA-dependent; maintenance of DNA methylation; negative regulation of gene expression, epigenetic; DNA methylation; gene expression; negative regulation of transcription from RNA polymerase II promoter; positive regulation of histone H3-K4 methylation; chromatin modification; gene silencing; regulation of gene expression, epigenetic; DNA methylation on cytosine; regulation of cell proliferation Disease: Cerebellar Ataxia, Deafness, And Narcolepsy, Autosomal Dominant; Neuropathy, Hereditary Sensory, Type Ie
NCBI Summary:	DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
UniProt Code:	P26358
NCBI GenInfo Identifier:	12231019
NCBI Gene ID:	1786
NCBI Accession:	P26358.2
UniProt Secondary Accession:	P26358,Q9UHG5, Q9ULA2, Q9UMZ6, A0AV63, B7ZLW6,
UniProt Related Accession:	P26358
Molecular Weight:	1616
NCBI Full Name:	DNA (cytosine-5)-methyltransferase 1
NCBI Synonym Full Names:	DNA (cytosine-5-)-methyltransferase 1
NCBI Official Symbol:	DNMT1
NCBI Official Synonym Symbols:	AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN
NCBI Protein Information:	DNA (cytosine-5)-methyltransferase 1; DNA (cytosine-5)-methyltransferase 1; m.HsaI; DNA MTase HsaI; DNA methyltransferase HsaI; CXXC-type zinc finger protein 9
UniProt Protein Name:	DNA (cytosine-5)-methyltransferase 1
UniProt Synonym Protein Names:	CXXC-type zinc finger protein 9; DNA methyltransferase HsaI; DNA MTase HsaI; M.HsaI; MCMT
UniProt Gene Name:	DNMT1
UniProt Entry Name:	DNMT1_HUMAN

	Western blot analysis of extracts of various cell lines, using DNMT1 antibody (CAB5495) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Western blot analysis of extracts from normal (control) and DNMT1 knockout (KO) 293T cells, using DNMT1 antibody (CAB5495) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of 293T cells using DNMT1 antibody (CAB5495) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug DNMT1 antibody (CAB5495). Western blot was performed from the immunoprecipitate using DNMT1 antibody (CAB5495) at a dilition of 1:1000.

Antibodies	ELISA Kits
Anti-DNMT1 Antibody (CAB16729)	Human DNMT1 ELISA Kit
Anti-Acetyl-DNMT1-K1127/K1129/K1131/K1133 Antibody (CAB5595)	Human DNA (cytosine-5)-methyltransferase 1 (DNMT1) ELISA Kit
Anti-Phospho-Dnmt1-pS714 Antibody (CABP0021)
Anti-Phospho-Dnmt1-pY399 Antibody (CABP0022)
Anti-DNMT1 Antibody (CAB16833)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)