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Immunohistochemistry analysis using Mouse Anti-DNMT1 Monoclonal Antibody, Clone 60B1220.1 (STMA00056). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-DNMT1 Monoclonal Antibody (STMA00056) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Magnification: 40x.
Key Features

Anti-DNMT1 Antibody [60B1220.1]

Datasheet
SKU STMA00056
Antibody Type Monoclonal
Applications WB
Applications IHC
Applications IP
Applications ChIP
Reactivity Human
Reactivity Mouse
Reactivity Fish
Reactivity Zebrafish (Danio rerio)
Host Species Mouse
Isotype IgG1 Kappa
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Description

DNMT1 Antibody [60B1220.1]

DNMT1 Antibody [60B1220.1] is a mouse monoclonal antibody directed against DNMT1, offered by Assay Genie for research applications. Reported applications include WB, IHC, IP and ChIP. Reported reactivity: Human, Mouse, Fish and Zebrafish (Danio rerio). Supplied as IgG1 Kappa, purified by Protein G.

DNMT1 (DNA methyltransferase 1) is the primary enzyme responsible for maintaining DNA methylation patterns during cell division. In the nervous system, DNMT1 regulates gene expression, synaptic plasticity, and neuronal differentiation through epigenetic mechanisms. Aberrant DNMT1 activity has been linked to neurodegenerative diseases, where altered methylation contributes to transcriptional silencing of neuroprotective genes and activation of pathogenic pathways. DNMT1 interacts with key regulatory proteins such as retinoblastoma (Rb), E2F1, and HDAC1, forming complexes that influence chromatin structure and gene repression. Loss of DNMT1 function leads to global hypomethylation, genomic instability, and impaired neuronal survival. Its role in epigenetic remodeling makes DNMT1 a promising target for therapeutic intervention and biomarker discovery in neurodegeneration. This antibody is also available conjugated to ATTO 390, ATTO 488, ATTO 594, APC, Biotin, FITC, HRP, PerCP and RPE. Supplied in 100 µg. For research use only; not for diagnostic or therapeutic procedures.

Product Name:DNMT1 Antibody [60B1220.1]
Product SKU:STMA00056
Size:100 µg
Target:DNMT1
Host Species:Mouse
Antibody Type:Monoclonal
Clone ID:60B1220.1
Research Areas:Cell Signaling, Post-translational Modifications, Organelle Markers
Buffer:PBS, 50% glycerol, 0.09% sodium azide
Storage & Shipping:Store at -20°C. Shipped on Blue Ice or 4°C. Conjugated variants should be stored according to the product label.
Format:Liquid
Applications:WB, IHC, IP, ChIP
Antibody Isotype:IgG1 Kappa
Purification:Protein G Purified
Concentration:1 mg/ml
Reactivity:Human, Mouse, Fish, Zebrafish (Danio rerio)
Recommended Dilution:WB (1:1000), IHC (1:1000); optimal dilutions for assays should be determined by the user.
Specificity:Detects ~180kDa. It will cross-react with mouse DNMT1.
Guarantee:12 months from date of dispatch
Immunogen:Raised against a synthetic peptide corresponding to amino acids 637-650 of human DNMT1
Immunogen Species:Human
Accession Number:NP_001370
Gene ID:1786
Swiss-Prot:P26358
Cellular Localization:Nucleus
Alternative Names:DNA Mtase, DNMT, MCMT, DNA methyltransferase 1, AIM, CXXC9, DNA (cytosine-5)-methyltransferase 1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI
References:1. Bestor T., et al.(1988) J.Mol. Biol. 203: 971-983. 2. Yen R.W., Vertino P.M., Nelkin B.D., et al. (1992) Nucl. Acids Res. 20: 2287-2291. 3. Xie S., et al. (1999) Gene 236: 87-95. 4. Okano M., Bell D.W., Haber D.A. and Li E. (1999) Cell 99: 247-257. 5. Reik W. et al. (1999) J. Nat. Genet 23: 380-382. 6. Robertson K.D., et al. (2000) Nat Genet 25(3): 338-342.
Note:For research use only.
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