The DOCK1 Antibody (CAB17359) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high reactivity with human samples and is specifically designed for use in Western blot applications. By binding to the Dock1 protein, this antibody enables precise detection and analysis in various cell types, making it an essential component for research in cell biology and cancer biology.Dock1, also known as dedicator of cytokinesis 1, plays a crucial role in cell migration, adhesion, and invasion, making it a key player in cancer progression and metastasis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
DOCK1 Antibody
SKU:
CAB17359
Size:
20μL, 100μL
Reactivity:
Human
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ced5, DOCK180, DOCK1
Positive Sample:
293T
Calculated MW:
215kDa
Observed MW:
215kDa
This gene encodes a member of the dedicator of cytokinesis protein family. Dedicator of cytokinesis proteins act as guanine nucleotide exchange factors for small Rho family G proteins. The encoded protein regulates the small GTPase Rac, thereby influencing several biological processes, including phagocytosis and cell migration. Overexpression of this gene has also been associated with certain cancers. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
1793
RRID
AB_2769208
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using DOCK1 Rabbit pAb (CAB17359) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
