The Donkey LH (Luteinizing Hormone) ELISA Kit offered by Assay Genie is a cutting-edge solution for the accurate measurement of luteinizing hormone levels in donkey serum, plasma, and other biological samples. This kit is meticulously designed to deliver exceptional sensitivity and specificity, ensuring precise and consistent results for various research applications. Luteinizing hormone is a key reproductive hormone that plays a crucial role in the regulation of the menstrual cycle and ovulation in females, as well as in the production of testosterone in males.
Monitoring luteinizing hormone levels is essential for understanding reproductive health and diagnosing hormonal imbalances in donkeys and other related species. With the Donkey LH ELISA Kit from Assay Genie, researchers can delve deeper into the intricate mechanisms of reproductive physiology in donkeys, paving the way for advancements in animal breeding, veterinary medicine, and hormonal research. This comprehensive kit is a valuable tool for researchers and professionals aiming to gain profound insights into the reproductive biology of donkeys.
Product Name:
Donkey LH (Luteinizing Hormone) ELISA Kit
SKU:
AEFI00057
Reactivity:
Donkey
Assay Type:
Competitive ELISA, Coated with Antigen
Sensitivity:
0.938 mIU/mL
Range:
1.563-100 mIU/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C(Sealed), Don't cryopreserve.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 10)
90-104%
92-100%
80-95%
EDTA Plasma (n = 10)
87-95%
84-101%
81-100%
Heparin Plasma (n = 10)
85-102%
88-103%
91-103%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 10)
85-101
94
EDTA Plasma (n = 10)
88-104
98
Heparin Plasma (n = 10)
93-101
98
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate TMB substrate for 30 minutes at room temperature. Prepare standards, samples (minimum 1:2 dilution), blanks, assign wells, and pre-wash the plate twice.
2
Sample & Biotin-Antibody Binding: Add 50 µL standard or sample followed by 50 µL biotin-labeled antibody to each well. Mix gently and incubate at 37°C for 45 minutes.
3
Washing: Wash the plate 5 times with wash buffer, allowing 1 minute soak time per wash.
4
Color Development: Add TMB substrate and incubate in the dark at 37°C for 10-20 minutes until color develops.
5
Stop Reaction: Add stop solution to terminate the reaction. The color changes from blue to yellow immediately.
6
Reading: Measure absorbance at 450 nm using a microplate reader.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant and store appropriately.
Plasma
Collect using EDTA anticoagulant, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Cell Culture Supernatant
Centrifuge at 1000 × g for 20 minutes at 4°C and collect clarified supernatant.
Cell Lysate
Lyse cells using recommended lysis buffer with protease inhibitors, centrifuge at 10,000 rpm for 10 minutes, and collect protein supernatant.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge at 5000 × g for 5 minutes, and collect supernatant.
Other Sample Types
Centrifuge samples at 1000 × g for 15 minutes at 2–8°C and collect supernatant. For additional guidance, please contact techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
