The Porcine LH (Luteinizing Hormone) ELISA Kit is meticulously designed to quantitatively detect luteinizing hormone levels in various porcine biological samples. Luteinizing hormone, a pivotal glycoprotein hormone secreted by the anterior pituitary gland, plays a vital role in reproductive functions, specifically in the regulation of ovulation and sex steroid hormone production. By accurately measuring LH levels, researchers can gain insights into the porcine reproductive cycle, ovulation timing, and reproductive health, making this ELISA kit an invaluable tool for studies related to porcine reproductive physiology and endocrinology. Assay Genie's Porcine LH ELISA Kit offers unparalleled sensitivity and specificity, ensuring precise and reproducible results. Manufactured under strict quality control standards, this kit guarantees robust performance and ease of use, providing researchers with a reliable and efficient method for investigating luteinizing hormone dynamics in porcine samples.
Product Name:
Porcine LH (Luteinizing Hormone) ELISA Kit
SKU:
AEES00278
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
