The E2F6 Antibody (CAB2718) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a member of a family of transcription factors that play a crucial role in the control of the cell cycle. The protein encoded by this gene lacks the transactivation and tumor suppressor protein association domains found in other family members, and contains a modular suppression domain that functions in the inhibition of transcription. It interacts in a complex with chromatin modifying factors. There are pseudogenes for this gene on chromosomes 22 and X. Alternative splicing results in multiple transcript variants. RRID AB_2764570 Gene ID 1876 Swiss Prot Synonym E2F-6; E2F6
This antibody is validated for use in WB, IF/ICC, IP, ChIP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
E2F6 Antibody
SKU:
CAB2718
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCIPChIPELISA
Recommended Dilution:
WB
1:500 - 1:2000
IF
/
ICC
1:50 - 1:200
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. Ch
IP
5μg antibody for 10μg-15μg of Chromatin
Synonyms:
E2F-6, E2F6
Positive Sample:
Mouse lung
Cellular Localization:
Nucleus.
Calculated MW:
32kDa
Observed MW:
35kDa
This gene encodes a member of a family of transcription factors that play a crucial role in the control of the cell cycle. The protein encoded by this gene lacks the transactivation and tumor suppressor protein association domains found in other family members, and contains a modular suppression domain that functions in the inhibition of transcription. It interacts in a complex with chromatin modifying factors. There are pseudogenes for this gene on chromosomes 22 and X. Alternative splicing results in multiple transcript variants. RRID AB_2764570 Gene ID 1876 Swiss Prot Synonym E2F-6; E2F6
Purification Method:
Affinity purification
Gene ID:
1876
RRID:
AB_2764570
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse lung using E2F6 Rabbit pAb (CAB2718) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunofluorescence analysis of U2OS cells using E2F6 Rabbit pAb (CAB2718). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis of extracts of 293T cells, using E2F6 antibody (CAB2718) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
