Anti-EED Antibody (CAB12773)

Antibody Name:	Anti-EED Antibody
Antibody SKU:	CAB12773
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human EED (NP_003788.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	HeLa, MCF7, A-549, K-562, Mouse liver, Mouse lung, Rat lung

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human EED (NP_003788.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSER EVST APAG TDMP AAKK QKLS SDEN SNPD LSGD ENDD AVSI ESGT NTER PDTP TNTP NAPG RKSW GKGK WKSK KCKY SFKC VNSL KEDH NQPL FGVQ
Gene ID:	8726
Uniprot:	O75530
Cellular Location:	Chromosome, Nucleus
Calculated MW:	45kDa/50kDa/53kDa
Observed MW:	50-75kDa

Synonyms:

EED, HEED, WAIT1

Background:

This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. Two transcript variants encoding distinct isoforms have been identified for this gene.

UniProt Protein Function:	Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
NCBI Summary:	This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	O75530
NCBI GenInfo Identifier:	205790383
NCBI Gene ID:	8726
NCBI Accession:	O75530.2
UniProt Secondary Accession:	O75530,O00149, Q6NTH2, Q7LDA5, Q7LDG8, Q86VV2, Q9UNY7 A8K7V5,
UniProt Related Accession:	O75530
Molecular Weight:	50kDa
NCBI Full Name:	Polycomb protein EED
NCBI Synonym Full Names:	embryonic ectoderm development
NCBI Official Symbol:	EED
NCBI Official Synonym Symbols:	HEED; WAIT1
NCBI Protein Information:	polycomb protein EED
UniProt Protein Name:	Polycomb protein EED
UniProt Synonym Protein Names:	Embryonic ectoderm development protein
Protein Family:	Polycomb protein
UniProt Gene Name:	EED

	Western blot analysis of extracts of various cell lines, using EED antibody (CAB12773) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Western blot analysis of extracts of various cell lines, using EED antibody (CAB12773) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunofluorescence analysis of L929 cells using EED Polyclonal Antibody (CAB12773) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using EED Polyclonal Antibody (CAB12773) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

Anti-EED Antibody (CAB12776)

Anti-EED Antibody (CAB5371)

EED Antibody (PACO08996)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)