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Anti-EEF2 Antibody (CAB0099)

SKU:
CAB0099
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
  • Metabolism Antibodies 1 Anti-EEF2 Antibody CAB0099
  • Metabolism Antibodies 1 Anti-EEF2 Antibody CAB0099
€139 - €419
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Description

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Antibody Name:Anti-EEF2 Antibody
Antibody SKU:CAB0099
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human EEF2 (NP_001952.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human
Positive Samples:293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human EEF2 (NP_001952.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MVNF TVDQ IRAI MDKK ANIR NMSV IAHV DHGK STLT DSLV CKAG IIAS ARAG ETRF TDTR KDEQ ERCI TIKS TAIS LFYE LSEN DLNF IKQS KDGA GFLI NLID SPGH VDFS SEVT AALR VTDG ALVV VDCV SGVC VQTE TVLR QAIA ERIK PVLM MNKM DRAL LELQ LEPE ELYQ TFQR IVEN VNVI ISTY GEGE SGPM GNIM IDPV LGTV GFGS GLHG WAFT LKQF AEMY VAKF AAKG EGQL GPAE RAKK VEDM MKKL WGDR YFDP ANGK FSKS ATSP EGKK LPRT FC
Gene ID:1938
Uniprot:P13639
Cellular Location:Cytoplasm, Nucleus
Calculated MW:95kDa
Observed MW:110kDa
Synonyms:EEF2, EEF-2, EF-2, EF2, SCA26
Background:This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation.
UniProt Protein Function:EEF2: a member of the GTP-binding translation elongation factor family. An essential factor for protein synthesis. Promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by eEF2 kinase phosphorylation. eEF2 kinase is normally dependent on Ca2+ ions and calmodulin. eEF2 kinase can also be activated by PKA in response to elevated cAMP levels, which are generally increased in stress- or starvation-related conditions. A variety of treatments known to raise intracellular Ca2+ or cAMP levels have been shown to result in increased phosphorylation of eEF2, and thus to inhibit peptide-chain elongation.
UniProt Protein Details:

Protein type:Translation elongation; Translation

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: polysome; membrane; cytoplasm; plasma membrane; cytosol; ribonucleoprotein complex; nucleus

Molecular Function:GTPase activity; protein binding; GTP binding; protein kinase binding; translation elongation factor activity

Biological Process: translational elongation; cellular protein metabolic process; translation; positive regulation of translation; peptidyl-diphthamide biosynthetic process from peptidyl-histidine; pathogenesis; hemopoietic progenitor cell differentiation; gene expression; post-translational protein modification

Disease: Spinocerebellar Ataxia 26

NCBI Summary:This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq, Jul 2008]
UniProt Code:P13639
NCBI GenInfo Identifier:119172
NCBI Gene ID:1938
NCBI Accession:P13639.4
UniProt Secondary Accession:P13639,Q58J86, B2RMP5, D6W618,
UniProt Related Accession:P13639
Molecular Weight:858
NCBI Full Name:Elongation factor 2
NCBI Synonym Full Names:eukaryotic translation elongation factor 2
NCBI Official Symbol:EEF2  
NCBI Official Synonym Symbols:EF2; EF-2; EEF-2; SCA26  
NCBI Protein Information:elongation factor 2; polypeptidyl-tRNA translocase
UniProt Protein Name:Elongation factor 2
Protein Family:Elongation factor
UniProt Gene Name:EEF2  
UniProt Entry Name:EF2_HUMAN
Anti-EEF2 Antibody (CAB0099)Western blot analysis of extracts of 293T cells, using EEF2 antibody (CAB0099) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-EEF2 Antibody (CAB0099)Immunofluorescence analysis of HeLa cells using EEF2 antibody (CAB0099). Blue: DAPI for nuclear staining.

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