Anti-EIF4G1 Antibody (CAB7552)

Antibody Name:	Anti-EIF4G1 Antibody
Antibody SKU:	CAB7552
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 400-500 of human EIF4G1 (NP_886553.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human
Positive Samples:	THP-1

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 400-500 of human EIF4G1 (NP_886553.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PEEL LNGA PSPP AVDL SPVS EPEE QAKE VTAS MAPP TIPS ATPA TAPS ATSP AQEE EMEE EEEE EEGE AGEA GEAE SEKG GEEL LPPE STPI PANL SQNL E
Gene ID:	1981
Uniprot:	Q04637
Cellular Location:
Calculated MW:	154kDa/158kDa/166kDa/171kDa/175kDa/176kDa
Observed MW:	230kDa

Synonyms:

EIF4G1, EIF-4G1, EIF4F, EIF4G, EIF4GI, P220, PARK18

Background:

The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage.

UniProt Protein Function:	eIF4G: Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with eIF3, mutually exclusive with EIF4A1 or EIFA2, EIF4E and through its N-terminus with PAPBC1. Interacts through its C-terminus with the serine/threonine kinases MKNK1, and with MKNK2. Appears to act as a scaffold protein, holding these enzymes in place to phosphorylate EIF4E. Non-phosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. EIF4G1/EIF4G3 interacts with PABPC1 to bring about circularization of the mRNA. Rapamycin can attenuate insulin stimulation mediated by FKBPs. Interacts with EIF4E3. Interacts with CIRBP and MIF4GD. Interacts with rotavirus A NSP3; in this interaction, NSP3 takes the place of PABPC1 thereby inducing shutoff of host protein synthesis. Interacts with RBM4. Belongs to the eIF4G family. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:RNA-binding; Translation; Translation initiation Chromosomal Location of Human Ortholog: 3q27.1 Cellular Component: membrane; eukaryotic translation initiation factor 4F complex; cytosol Molecular Function:protein binding; translation factor activity, nucleic acid binding; translation initiation factor activity Biological Process: poly(A) tail shortening; cellular protein metabolic process; translation; viral reproduction; cytokine and chemokine mediated signaling pathway; translational initiation; insulin receptor signaling pathway; mRNA catabolic process, nonsense-mediated decay; gene expression; regulation of translational initiation; mRNA catabolic process, deadenylation-dependent decay
NCBI Summary:	The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage. [provided by RefSeq, Aug 2010]
UniProt Code:	Q04637
NCBI GenInfo Identifier:	294862538
NCBI Gene ID:	1981
NCBI Accession:	Q04637.4
UniProt Secondary Accession:	Q04637,O43177, O95066, Q5HYG0, Q6ZN21, Q8N102, D3DNT2 D3DNT4, D3DNT5, E9PFM1, G5E9S1,
UniProt Related Accession:	Q04637
Molecular Weight:	175,491 Da
NCBI Full Name:	Eukaryotic translation initiation factor 4 gamma 1
NCBI Synonym Full Names:	eukaryotic translation initiation factor 4 gamma, 1
NCBI Official Symbol:	EIF4G1
NCBI Official Synonym Symbols:	P220; EIF4F; EIF4G; EIF4GI; PARK18; EIF-4G1
NCBI Protein Information:	eukaryotic translation initiation factor 4 gamma 1; EIF4-gamma; eIF-4-gamma 1; eucaryotic translation initiation factor 4G
UniProt Protein Name:	Eukaryotic translation initiation factor 4 gamma 1
UniProt Synonym Protein Names:	p220
Protein Family:	Eukaryotic translation initiation factor
UniProt Gene Name:	EIF4G1
UniProt Entry Name:	IF4G1_HUMAN

	Western blot analysis of extracts of THP-1 cells, using EIF4G1 Antibody (CAB7552) at 1:200 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human liver damage using EIF4G1 Antibody (CAB7552) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver cancer using EIF4G1 Antibody (CAB7552) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human breast cancer using EIF4G1 Antibody (CAB7552) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A549 cells using EIF4G1 antibody (CAB7552). Blue: DAPI for nuclear staining.

Antibodies

Anti-EIF4G1 Antibody (CAB0881)

Anti-EIF4G Antibody (CAB6086)

Anti-Phospho-EIF4G1-S1108 pAb Antibody (CABP0796)

Phospho-EIF4G1 (S1148) Antibody (PACO03602)

EIF4G1 Antibody (PACO03603)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)