Anti-EIF4G2 Antibody (CAB2897)

Antibody Name:	Anti-EIF4G2 Antibody
Antibody SKU:	CAB2897
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 750-850 of human EIF4G2 (NP_001409.3).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:100 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, SW480, A-549, BT-474, 293T, SH-SY5Y, Mouse kidney, Mouse heart

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 750-850 of human EIF4G2 (NP_001409.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	IYKW IKDN ISPK LHVD KGFV NILM TSFL QYIS SEVN PPSD ETDS SSAP SKEQ LEQE KQLL LSFK PVMQ KFLH DHVD LQVS ALYA LQVH CYNS NFPK GMLL R
Gene ID:	1982
Uniprot:	P78344
Cellular Location:
Calculated MW:	98kDa/102kDa
Observed MW:	102kDa

Synonyms:

EIF4G2, AAG1, DAP5, NAT1, P97

Background:

Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.

UniProt Protein Function:	EIF4G2: a general repressor of translation by forming translationally inactive complexes. Appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Interacts with the protein kinases Mnk1 and -2. Binds EIF4A and EIF3. Cleaved by some caspases and viral proteases.
UniProt Protein Details:	Protein type:RNA-binding; Translation initiation; Translation Chromosomal Location of Human Ortholog: 11p15 Cellular Component: membrane; eukaryotic translation initiation factor 4F complex; cytosol Molecular Function:protein binding; translation factor activity, nucleic acid binding; translation initiation factor activity Biological Process: cell death; cytokine and chemokine mediated signaling pathway; translational initiation; cell cycle arrest; regulation of translational initiation
NCBI Summary:	Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008]
UniProt Code:	P78344
NCBI GenInfo Identifier:	30315906
NCBI Gene ID:	1982
NCBI Accession:	P78344.1
UniProt Secondary Accession:	P78344,O60877, P78404, Q0VH00, Q0VH01, Q2NKW9, Q49A79 Q53EU1, Q58EZ2, Q8NI71, Q96C16,
UniProt Related Accession:	P78344
Molecular Weight:	907
NCBI Full Name:	Eukaryotic translation initiation factor 4 gamma 2
NCBI Synonym Full Names:	eukaryotic translation initiation factor 4 gamma, 2
NCBI Official Symbol:	EIF4G2
NCBI Official Synonym Symbols:	P97; AAG1; DAP5; NAT1
NCBI Protein Information:	eukaryotic translation initiation factor 4 gamma 2; DAP-5; EIF4G2; eIF4G 2; eIF-4G 2; eIF-4-gamma 2; aging-associated protein 1; death-associated protein 5; eukaryotic translation initiation factor 4G-like 1
UniProt Protein Name:	Eukaryotic translation initiation factor 4 gamma 2
UniProt Synonym Protein Names:	Death-associated protein 5; DAP-5; p97
Protein Family:	Eukaryotic translation initiation factor
UniProt Gene Name:	EIF4G2
UniProt Entry Name:	IF4G2_HUMAN

	Western blot analysis of extracts of various cell lines, using EIF4G2 antibody (CAB2897) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse brain using EIF4G2 Rabbit pAb (CAB2897) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using EIF4G2 Rabbit pAb (CAB2897) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of HeLa cells using EIF4G2 Rabbit pAb (CAB2897) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH-3T3 cells using EIF4G2 Rabbit pAb (CAB2897) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

Anti-EIF4G2 Antibody (CAB13616)

Anti-EIF4G2 Antibody (CAB19990)[KO Validated]

EIF4G2 Antibody (PACO06419)

EIF4G2 Antibody (PACO09058)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)