The ELMO2 Antibody (CAB13785) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to Elmo2 and is suitable for use in various applications such as Western blot and immunofluorescence.Elmo2 is known to interact with Dock180, forming an important complex involved in Rac GTPase activation and cytoskeletal rearrangements that are essential for cell migration and phagocytosis processes. By targeting Elmo2, researchers can gain insights into the mechanisms underlying these cellular functions and potentially uncover new therapeutic targets for diseases involving abnormal cell migration or phagocytosis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ELMO2 Antibody
SKU:
CAB13785
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
VMPI, CED12, CED-12, ELMO-2, Ced-12A, ELMO2
Positive Sample:
HeLa, Mouse brain, Rat brain
Cellular Localization:
Cytoplasm, Membrane, Cytosol.
Calculated MW:
83kDa
Observed MW:
83kDa
The protein encoded by this gene interacts with the dedicator of cyto-kinesis 1 protein. Similarity to a C. elegans protein suggests that this protein may function in phagocytosis of apoptotic cells and in cell migration. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
63916
RRID
AB_2760643
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ELMO2 Rabbit pAb (CAB13785) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
