Anti-EMB Antibody (CAB10423)

SKU:
CAB10423
€99 - €249

Description

Technical Manual

Anti-EMB Antibody

Product Name:Anti-EMB Antibody
Product SKU:CAB10423
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

This gene encodes a transmembrane glycoprotein that is a member of the immunoglobulin superfamily. The encoded protein may be involved in cell growth and development by mediating interactions between the cell and extracellular matrix. A pseudogene of this gene is found on chromosome 1.

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 33-260 of human EMB (NP_940851.1).
Sequence:

DGSAPDSPFTSPPLREEIMANNFSLESHNISLTEHSSMPVEKNITLERPSNVNLTCQFTTSGDLNAVNVTWKKDGEQLENNYLVSATGSTLYTQYRFTIINSKQMGSYSCFFREEKEQRGTFNFKVPELHGKNKPLISYVGDSTVLTCKCQNCFPLNWTWYSSNGSVKVPVGVQMNKYVINGTYANETKLKITQLLEEDGESYWCRALFQLGESEEHIELVVLSYLVP

Gene ID:133418
Swiss Prot:Q6PCB8
Synonyms:EMB; GP70; embigin
Calculated MW:31kDa/36kDa
Observed MW:37kDa
Reactivity:Human
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting
Positive Samples:Jurkat, MCF7, THP-1
Cellular Location:Cell junction, Cell membrane, Single-pass type I membrane protein, synapse

Product Images

Anti-EMB Antibody (CAB10423)

Western blot analysis of extracts of various cell lines, using EMB antibody (CAB10423) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

Uniprot Information

UniProt Protein Function:EMB: May function as a regulator of cell/ECM interactions during development and in the homeostasis of normal adult tissues.
UniProt Protein Details:

Protein type:Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q11.1

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function:monocarboxylic acid transmembrane transporter activity

NCBI Summary:This gene encodes a transmembrane glycoprotein that is a member of the immunoglobulin superfamily. The encoded protein may be involved in cell growth and development by mediating interactions between the cell and extracellular matrix. A pseudogene of this gene is found on chromosome 1. [provided by RefSeq, Jan 2009]
UniProt Code:Q6PCB8
NCBI GenInfo Identifier:83286878
NCBI Gene ID:133418
NCBI Accession:Q6PCB8.1
UniProt Secondary Accession:Q6PCB8,B7Z6S3, B7Z902,
UniProt Related Accession:Q6PCB8
Molecular Weight:37kDa
NCBI Full Name:Embigin
NCBI Synonym Full Names:embigin
NCBI Official Symbol:EMB
NCBI Official Synonym Symbols:GP70
NCBI Protein Information:embigin
UniProt Protein Name:Embigin
Protein Family:Embigin
UniProt Gene Name:EMB
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Additional Information

Product type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
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