The EN2 Antibody (CAB17480) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit polyclonal antibody is highly specific and reactive with human samples, making it ideal for studying EN2 expression and localization in various tissues and cell types.EN2 is involved in the regulation of gene expression during embryonic development, particularly in the formation of the midbrain and cerebellum. Dysregulation of EN2 has been linked to neurodevelopmental disorders such as autism spectrum disorder and schizophrenia, making it a key target for research in neurobiology and neuropsychiatry.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
EN2 Antibody
SKU:
CAB17480
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
EN2
Positive Sample:
U-87MG, Mouse brain, Rat brain
Cellular Localization:
Fibrillar Center, Nucleolus, Nucleoplasm, Nucleus.
Calculated MW:
34kDa
Observed MW:
33kDa
Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.
Purification Method
Affinity purification
Gene ID
2020
RRID
AB_2769319
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using EN2 Rabbit pAb (CAB17480) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
