Anti-ENO3 Antibody (CAB16371)

Antibody Name:	Anti-ENO3 Antibody
Antibody SKU:	CAB16371
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human ENO3 (NP_001967.3).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse heart, Mouse skeletal muscle, Rat heart, Rat skeletal muscle

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human ENO3 (NP_001967.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAMQ KIFA REIL DSRG NPTV EVDL HTAK GRFR AAVP SGAS TGIY EALE LRDG DKGR YLGK GVLK AVEN INNT LGPA LLQK KLSV VDQE KVDK FMIE LDGT ENKS KFGA NAIL GVSL AVCK AGAA EKGV PLYR HIAD LAGN PDLI LPVP AFNV INGG SHAG NKLA MQEF MILP VGAS SFKE AMRI GAEV YH
Gene ID:	2027
Uniprot:	P13929
Cellular Location:	Cytoplasm
Calculated MW:	42kDa/44kDa/46kDa
Observed MW:	47kDa

Synonyms:

ENO3, GSD13, MSE, enolase 3

Background:

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme is found in skeletal muscle cells in the adult where it may play a role in muscle development and regeneration. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene have be associated glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been described.

UniProt Protein Function:	ENO3: Appears to have a function in striated muscle development and regeneration. Defects in ENO3 are the cause of glycogen storage disease type 13 (GSD13). A metabolic disorder that results in exercise-induced myalgias, generalized muscle weakness and fatigability. It is characterized by increased serum creatine kinase and decreased enolase 3 activity. Dramatically reduced protein levels with focal sarcoplasmic accumulation of glycogen- beta particles are detected on ultrastructural analysis. Belongs to the enolase family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Lyase; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 4.2.1.11 Chromosomal Location of Human Ortholog: 17p13.2 Cellular Component: extracellular space; phosphopyruvate hydratase complex; cytoplasm; plasma membrane; cytosol Molecular Function:protein homodimerization activity; protein heterodimerization activity; magnesium ion binding; phosphopyruvate hydratase activity Biological Process: response to drug; glycolysis; carbohydrate metabolic process; skeletal muscle regeneration; glucose metabolic process; pathogenesis; gluconeogenesis; aging Disease: Glycogen Storage Disease Xiii
NCBI Summary:	This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme is found in skeletal muscle cells in the adult where it may play a role in muscle development and regeneration. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene have be associated glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Jul 2010]
UniProt Code:	P13929
NCBI GenInfo Identifier:	425906077
NCBI Gene ID:	2027
NCBI Accession:	P13929.5
UniProt Related Accession:	P13929
Molecular Weight:
NCBI Full Name:	Beta-enolase
NCBI Synonym Full Names:	enolase 3
NCBI Official Symbol:	ENO3
NCBI Official Synonym Symbols:	MSE; GSD13
NCBI Protein Information:	beta-enolase
UniProt Protein Name:	Beta-enolase
UniProt Synonym Protein Names:	2-phospho-D-glycerate hydro-lyase; Enolase 3; Muscle-specific enolase; MSE; Skeletal muscle enolase
Protein Family:	Enolase
UniProt Gene Name:	ENO3
UniProt Entry Name:	ENOB_HUMAN

	Western blot analysis of extracts of various cell lines, using ENO3 Rabbit pAb (CAB16371) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of HeLa cells using ENO3 antibody (CAB16371) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH/3T3 cells using ENO3 antibody (CAB16371) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of PC-12 cells using ENO3 antibody (CAB16371) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Anti-ENO3 Antibody (CAB3852)

ENO3 Antibody (PACO09092)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)