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Anti-ERAP1 Antibody (CAB5248)

SKU:
CAB5248
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
€139 - €419
Frequently bought together:

Description

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Antibody Name:Anti-ERAP1 Antibody
Antibody SKU:CAB5248
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 642-941 of human ERAP1 (NP_001185470.1).
Application:WB
Recommended Dilution:WB 1:1000 - 1:2000
Reactivity:Human, Mouse
Positive Samples:293T, LO2
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 642-941 of human ERAP1 (NP_001185470.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:NAFQ LVSI GKLS IEKA LDLS LYLK HETE IMPV FQGL NELI PMYK LMEK RDMN EVET QFKA FLIR LLRD LIDK QTWT DEGS VSER MLRS QLLL LACV HNYQ PCVQ RAEG YFRK WKES NGNL SLPV DVTL AVFA VGAQ STEG WDFL YSKY QFSL SSTE KSQI EFAL CRTQ NKEK LQWL LDES FKGD KIKT QEFP QILT LIGR NPVG YPLA WQFL RKNW NKLV QKFE LGSS SIAH MVMG TTNQ FSTR TRLE EVKG FFSS LKEN GSQL RCVQ QTIE TIEE NIGW MDKN FDKI RVWL QSEK LERM
Gene ID:51752
Uniprot:Q9NZ08
Cellular Location:Endoplasmic reticulum membrane, Single-pass type II membrane protein
Calculated MW:107kDa
Observed MW:107kDa
Synonyms:ERAP1, A-LAP, ALAP, APPILS, ARTS-1, ARTS1, ERAAP, ERAAP1, PILS-AP, PILSAP
Background:The protein encoded by this gene is an aminopeptidase involved in trimming HLA class I-binding precursors so that they can be presented on MHC class I molecules. The encoded protein acts as a monomer or as a heterodimer with ERAP2. This protein may also be involved in blood pressure regulation by inactivation of angiotensin II. Three transcript variants encoding two different isoforms have been found for this gene.
UniProt Protein Function:ARTS1: Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I- binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney. Belongs to the peptidase M1 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Protease; EC 3.4.11.-; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q15

Cellular Component: endoplasmic reticulum lumen; extracellular region; extracellular space; membrane; plasma membrane

Molecular Function:aminopeptidase activity; endopeptidase activity; interleukin-1, Type II receptor binding; interleukin-6 receptor binding; metalloexopeptidase activity; peptide binding; protein binding; zinc ion binding

Biological Process: angiogenesis; antigen processing and presentation of endogenous peptide antigen via MHC class I; antigen processing and presentation of peptide antigen via MHC class I; membrane protein ectodomain proteolysis; peptide catabolic process; regulation of blood pressure

NCBI Summary:The protein encoded by this gene is an aminopeptidase involved in trimming HLA class I-binding precursors so that they can be presented on MHC class I molecules. The encoded protein acts as a monomer or as a heterodimer with ERAP2. This protein may also be involved in blood pressure regulation by inactivation of angiotensin II. Three transcript variants encoding two different isoforms have been found for this gene.[provided by RefSeq, Oct 2010]
UniProt Code:Q9NZ08
NCBI GenInfo Identifier:158937334
NCBI Gene ID:51752
NCBI Accession:Q9NZ08.3
UniProt Secondary Accession:Q9NZ08,O60278, Q6UWY6, Q8NEL4, Q8TAD0, Q9UHF8, Q9UKY2
UniProt Related Accession:Q9NZ08
Molecular Weight:107,841 Da
NCBI Full Name:Endoplasmic reticulum aminopeptidase 1
NCBI Synonym Full Names:endoplasmic reticulum aminopeptidase 1
NCBI Official Symbol:ERAP1  
NCBI Official Synonym Symbols:ALAP; A-LAP; ARTS1; ERAAP; APPILS; ARTS-1; ERAAP1; PILSAP; PILS-AP  
NCBI Protein Information:endoplasmic reticulum aminopeptidase 1
UniProt Protein Name:Endoplasmic reticulum aminopeptidase 1
UniProt Synonym Protein Names:ARTS-1; Adipocyte-derived leucine aminopeptidase; A-LAP; Aminopeptidase PILS; Puromycin-insensitive leucyl-specific aminopeptidase; PILS-AP; Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator
Protein Family:Endoplasmic reticulum aminopeptidase
UniProt Gene Name:ERAP1  
UniProt Entry Name:ERAP1_HUMAN
Anti-ERAP1 Antibody (CAB5248)Western blot analysis of extracts of various cell lines, using ERAP1 antibody (CAB5248) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

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