Anti-ESYT1 Antibody (CAB15410)
- SKU:
- CAB15410
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-ESYT1 Antibody |
| Antibody SKU: | CAB15410 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 90-220 of human ESYT1 (NP_056107.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:200 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-251MG, 293T, LO2, Jurkat, HeLa, Mouse brain, Mouse heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 90-220 of human ESYT1 (NP_056107.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GWRR VRDE KERS LRAA RQLL DDEE QLTA KTLY MSHR ELPA WVSF PDVE KAEW LNKI VAQV WPFL GQYM EKLL AETV APAV RGSN PHLQ TFTF TRVE LGEK PLRI IGVK VHPG QRKE QILL DLNI SYVG DVQ |
| Gene ID: | 23344 |
| Uniprot: | Q9BSJ8 |
| Cellular Location: | Cell membrane, Endoplasmic reticulum membrane, Multi-pass membrane protein, Peripheral membrane protein |
| Calculated MW: | 122kDa/124kDa |
| Observed MW: | 123kDa |
| Synonyms: | ESYT1, FAM62A, MBC2 |
| Background: |
| UniProt Protein Function: | ESYT1: May play a role as calcium-regulated intrinsic membrane protein. Belongs to the extended synaptotagmin family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 12q13.2 Cellular Component: integral to endoplasmic reticulum membrane; membrane Molecular Function:protein binding |
| UniProt Code: | Q9BSJ8 |
| NCBI GenInfo Identifier: | 74733019 |
| NCBI Gene ID: | 23344 |
| NCBI Accession: | Q9BSJ8.1 |
| UniProt Secondary Accession: | Q9BSJ8,O94848, Q6PJN4, Q9H6J1, Q9H6W2, Q9Y416, A0FGR7 A8K2S2, |
| UniProt Related Accession: | Q9BSJ8 |
| Molecular Weight: | 124,003 Da |
| NCBI Full Name: | Extended synaptotagmin-1 |
| NCBI Synonym Full Names: | extended synaptotagmin 1 |
| NCBI Official Symbol: | ESYT1Â Â |
| NCBI Official Synonym Symbols: | MBC2; FAM62AÂ Â |
| NCBI Protein Information: | extended synaptotagmin-1 |
| UniProt Protein Name: | Extended synaptotagmin-1 |
| UniProt Synonym Protein Names: | Membrane-bound C2 domain-containing protein |
| Protein Family: | Extended synaptotagmin |
| UniProt Gene Name: | ESYT1Â Â |
| UniProt Entry Name: | ESYT1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ESYT1 antibody (CAB15410) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunofluorescence analysis of U-2 OS cells using ESYT1 antibody (CAB15410) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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