The EXOSC2 Antibody (CAB10450) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in various applications including Western blotting. By specifically recognizing the Exosc2 protein, this antibody enables detailed analysis and detection of Exosc2 expression in different cell types.Exosc2, also known as RRP4, plays a vital role in maintaining RNA quality control and regulating gene expression. Dysregulation of Exosc2 has been linked to various diseases including cancer, neurodegenerative disorders, and developmental abnormalities.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
EXOSC2 Antibody
SKU:
CAB10450
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
p7, RRP4, SHRF, Rrp4p, hRrp4p, EXOSC2
Positive Sample:
A-549, HeLa, Mouse liver, Mouse spleen, Rat liver
Cellular Localization:
Cytoplasm, Nucleus, Nucleolus.
Calculated MW:
33kDa
Observed MW:
33kDa
Predicted to enable RNA binding activity. Involved in positive regulation of cell growth. Located in cytoplasm; nucleolus; and nucleoplasm. Part of nuclear exosome (RNase complex).
Purification Method
Affinity purification
Gene ID
23404
RRID
AB_2757998
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using EXOSC2 Rabbit pAb (CAB10450) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Immunofluorescence analysis of U2OS cells using EXOSC2 Rabbit pAb (CAB10450) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of H9C2 cells using EXOSC2 Rabbit pAb (CAB10450) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
