The EXTL3 Antibody (CAB3857) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the EXTL3 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an ideal choice for studies in glycobiology and cancer research.EXTL3 plays a critical role in the modification of heparan sulfate chains, which are essential components of the extracellular matrix and play key roles in cell signaling and adhesion.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
EXTL3 Antibody
SKU:
CAB3857
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RPR, BOTV, REGR, EXTR1, ISDNA, EXTL1L, EXTL3
Positive Sample:
293T, Mouse pancreas, Rat lung
Cellular Localization:
Endoplasmic Reticulum Membrane, Single-Pass Type Ii Membrane Protein.
Calculated MW:
105kDa
Observed MW:
105kDa
This gene encodes a single-pass membrane protein which functions as a glycosyltransferase. The encoded protein catalyzes the transfer of N-acetylglucosamine to glycosaminoglycan chains. This reaction is important in heparin and heparan sulfate synthesis. Alternative splicing results in the multiple transcript variants.
Purification Method
Affinity purification
Gene ID
2137
RRID
AB_2765345
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using EXTL3 Rabbit pAb (CAB3857) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Immunohistochemistry analysis of paraffin-embedded Rat kidney using EXTL3 Rabbit pAb (CAB3857) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
