The Exportin 5 (XPO5) Monoclonal Antibody (CAB3813) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using rabbit monoclonal technology, exhibits high specificity and sensitivity towards Exportin-5 in various experimental settings. Validated for use in applications such as immunofluorescence and immunoprecipitation, it offers robust performance for detecting and studying Exportin-5 dynamics in cells.Exportin-5 plays a crucial role in the transport of RNA molecules from the nucleus to the cytoplasm, making it a central player in gene expression regulation.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Exportin 5 (XPO5) Monoclonal Antibody
SKU:
CAB3813
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0838
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
exp5, Exportin 5 (XPO5)
Positive Sample:
U-2 OS, MCF7, U-251MG，Mouse brain, Mouse kidney, Rat brain
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
136kDa
Observed MW:
136kDa
This gene encodes a member of the karyopherin family that is required for the transport of small RNAs and double-stranded RNA-binding proteins from the nucleus to the cytoplasm. The encoded protein translocates cargo through the nuclear pore complex in a RanGTP-dependent process.
Purification Method
Affinity purification
Gene ID
57510
RRID
AB_2863144
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Exportin 5 (XPO5) (XPO5) Rabbit mAb (CAB3813) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Western blot analysis of various lysates using Exportin 5 (XPO5) (XPO5) Rabbit mAb (CAB3813) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
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