Anti-XPO5 Antibody (CAB6790)

Antibody Name:	Anti-XPO5 Antibody
Antibody SKU:	CAB6790
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 925-1204 of human XPO5 (NP_065801.1).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, U-251MG, NIH/3T3, HeLa, PC-12, Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 925-1204 of human XPO5 (NP_065801.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MRLS QKWQ VINQ RSLL CGED EAAD ENPE SQEM LEEQ LVRM LTRE VMDL ITVC CVSK KGAD HSSA PPAD GDDE EMMA TEVT PSAM AELT DLGK CLMK HEDV CTAL LITA FNSL AWKD TLSC QRTT SQLC WPLL KQVL SGTL LADA VTWL FTSV LKGL QMHG QHDG CMAS LVHL AFQI YEAL RPRY LEIR AVME QIPE IQKD SLDQ FDCK LLNP SLQK VADK RRKD QFKR LIAG CIGK PLGE QFRK EVHI KNLP SLFK KTKP MLET EVLD NDGG GLAT IFEP
Gene ID:	57510
Uniprot:	Q9HAV4
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	136kDa
Observed MW:	125kDa

Synonyms:	XPO5, exp5
Background:	This gene encodes a member of the karyopherin family that is required for the transport of small RNAs and double-stranded RNA-binding proteins from the nucleus to the cytoplasm. The encoded protein translocates cargo through the nuclear pore complex in a RanGTP-dependent process.

UniProt Protein Function:	Exportin-5: mediates the nuclear export of proteins bearing double-stranded RNA binding domains and double-stranded RNA. Overexpression enhances RNA interference mediated by shRNAs and miRNAs. In the nucleus it binds cooperatively to its cargo and to Ran in its active GTP-bound form. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause disassembly of the complex and release of the cargo from the export receptor. Exp5 then returns to the nuclear compartment by diffusion through the nuclear pore complex, to mediate another round of transport. Two isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Nuclear export; Karyopherin; Nuclear import; RNA-binding Chromosomal Location of Human Ortholog: 6p21.1 Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus Molecular Function:mRNA binding; protein binding; Ran GTPase binding; RNA binding Biological Process: pre-microRNA export from nucleus; protein export from nucleus; RNA-mediated gene silencing
NCBI Summary:	This gene encodes a member of the karyopherin family that is required for the transport of small RNAs and double-stranded RNA-binding proteins from the nucleus to the cytoplasm. The encoded protein translocates cargo through the nuclear pore complex in a RanGTP-dependent process. [provided by RefSeq, Aug 2011]
UniProt Code:	Q9HAV4
NCBI GenInfo Identifier:	74734245
NCBI Gene ID:	57510
NCBI Accession:	Q9HAV4.1
UniProt Secondary Accession:	Q9HAV4,Q5JTE6, Q96G48, Q96HN3, Q9BWM6, Q9BZV5, Q9H9M4 Q9NT89, Q9NW39, Q9ULC9,
UniProt Related Accession:	Q9HAV4
Molecular Weight:	136,311 Da
NCBI Full Name:	Exportin-5
NCBI Synonym Full Names:	exportin 5
NCBI Official Symbol:	XPO5
NCBI Official Synonym Symbols:	exp5
NCBI Protein Information:	exportin-5
UniProt Protein Name:	Exportin-5
UniProt Synonym Protein Names:	Ran-binding protein 21
Protein Family:	Exportin
UniProt Gene Name:	XPO5
UniProt Entry Name:	XPO5_HUMAN

	Western blot analysis of extracts of various cell lines, using XPO5 antibody (CAB6790). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human oophoroma using XPO5 antibody (CAB6790) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse jejunum using XPO5 antibody (CAB6790) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of MCF7 cells using XPO5 antibody (CAB6790).
	Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug XPO5 antibody (CAB6790). Western blot was performed from the immunoprecipitate using XPO5 antibody (CAB6790) at a dilition of 1:1000.

Antibodies

Anti-Exportin 5 Antibody (CAB3813)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)