Anti-F3 Antibody (CAB1378)

Antibody Name:	Anti-F3 Antibody
Antibody SKU:	CAB1378
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 120-251 of human F3 (NP_001984.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Rat
Positive Samples:	NCI-H460, SW480, HL-60, SKOV3

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 120-251 of human F3 (NP_001984.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SAGE PLYE NSPE FTPY LETN LGQP TIQS FEQV GTKV NVTV EDER TLVR RNNT FLSL RDVF GKDL IYTL YYWK SSSS GKKT AKTN TNEF LIDV DKGE NYCF SVQA VIPS RTVN RKST DSPV ECMG QEKG EFRE
Gene ID:	2152
Uniprot:	P13726
Cellular Location:	Membrane, Secreted, Single-pass type I membrane protein
Calculated MW:	27kDa/33kDa
Observed MW:	45kDa

Synonyms:

F3, CD142, TF, TFA

Background:

This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.

UniProt Protein Function:	F3: Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. Interacts with HSPE; the interaction, inhibited by heparin, promotes the generation of activated factor X and activates coagulation in the presence of activated factor VII. TF expression is highly dependent upon cell type. TF can also be induced by the inflammatory mediators interleukin 1 and TNF-alpha, as well as by endotoxin, to appear on monocytes and vascular endothelial cells as a component of cellular immune response. Lung, placenta and pancreas. Belongs to the tissue factor family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral; Cell surface Chromosomal Location of Human Ortholog: 1p22-p21 Cellular Component: cell surface; cytoplasm; extracellular matrix; extracellular space; integral to membrane; plasma membrane Molecular Function:hematopoietin/interferon-class (D200-domain) cytokine receptor activity; phospholipid binding; protease binding; protein binding Biological Process: activation of blood coagulation via clotting cascade; activation of plasma proteins during acute inflammatory response; aging; blood coagulation; blood coagulation, extrinsic pathway; caspase activation; cytokine and chemokine mediated signaling pathway; positive regulation of angiogenesis; positive regulation of cell migration; positive regulation of endothelial cell proliferation; positive regulation of positive chemotaxis; positive regulation of protein kinase B signaling cascade; positive regulation of smooth muscle cell migration; response to estradiol stimulus; response to lipopolysaccharide; response to low density lipoprotein stimulus; response to mechanical stimulus; response to temperature stimulus
NCBI Summary:	This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
UniProt Code:	P13726
NCBI GenInfo Identifier:	135666
NCBI Gene ID:	2152
NCBI Accession:	P13726.1
UniProt Secondary Accession:	P13726,Q6FHG2, Q86WH4, D3DT47,
UniProt Related Accession:	P13726
Molecular Weight:	27,145 Da
NCBI Full Name:	Tissue factor
NCBI Synonym Full Names:	coagulation factor III, tissue factor
NCBI Official Symbol:	F3
NCBI Official Synonym Symbols:	TF; TFA; CD142
NCBI Protein Information:	tissue factor
UniProt Protein Name:	Tissue factor
UniProt Synonym Protein Names:	Coagulation factor III; Thromboplastin; CD_antigen: CD142
Protein Family:	Tissue factor
UniProt Gene Name:	F3
UniProt Entry Name:	TF_HUMAN

	Western blot analysis of extracts of various cell lines, using F3 antibody (CAB1378) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded rat liver using F3 Antibody (CAB1378) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophagus using F3 Antibody (CAB1378) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-Tissue Factor Antibody (CAB4395)	Human Tissue factor (F3) ELISA Kit
F3 Antibody (PACO01608)	Coagulation Factor III Colorimetric Cell-Based ELISA Kit
F3 Antibody (PACO01907)	Coagulation Factor III (Phospho-Ser290) Colorimetric Cell-Based ELISA Kit
Phospho-F3 (S290) Antibody (PACO03647)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)