The FABP4 Antibody (CAB0232) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in applications such as Western blotting. By binding to FABP4, it enables the detection and analysis of this protein in various cell types, making it ideal for research in fields such as metabolic disorders, cardiovascular disease, and obesity.FABP4, also known as adipocyte fatty acid-binding protein, is a key player in regulating lipid uptake and storage in adipocytes, as well as in promoting inflammation in various tissues.
This antibody is validated for use in WB, IHC-P, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
FABP4 Antibody
SKU:
CAB0232
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
aP2, ALBP, AFABP, A-FABP, HEL-S-104, FABP4
Positive Sample:
RT-4, Mouse kidney, Mouse heart, Rat heart
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
15kDa
Observed MW:
14kDa
FABP4 encodes the fatty acid binding protein found in adipocytes. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism.
Purification Method
Affinity purification
Gene ID
2167
RRID
AB_2757045
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using FABP4 Rabbit pAb (CAB0232) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Western blot analysis of various lysates, using FABP4 Rabbit pAb (CAB0232) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Immunofluorescence analysis of paraffin-embedded Rat adipose using FABP4 Rabbit pAb (CAB0232) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
