Anti-FBL Antibody (CAB13490)

Antibody Name:	Anti-FBL Antibody
Antibody SKU:	CAB13490
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat, Monkey
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 100-321 of human FBL (NP_001427.2).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:20 - 1:50
Reactivity:	Human, Mouse, Rat, Monkey
Positive Samples:	HeLa, A375, U-2OS, COS-1, COS-7, PC-12, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 100-321 of human FBL (NP_001427.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	RGKE DALV TKNL VPGE SVYG EKRV SISE GDDK IEYR AWNP FRSK LAAA ILGG VDQI HIKP GAKV LYLG AASG TTVS HVSD IVGP DGLV YAVE FSHR SGRD LINL AKKR TNII PVIE DARH PHKY RMLI AMVD VIFA DVAQ PDQT RIVA LNAH TFLR NGGH FVIS IKAN CIDS TASA EAVF ASEV KKMQ QENM KPQE QLTL EPYE RDHA VVVG VYRP PPKV KN
Gene ID:	2091
Uniprot:	P22087
Cellular Location:	Nucleus, nucleolus
Calculated MW:	33kDa
Observed MW:	37kDa

Synonyms:

FBL, FIB, FLRN, RNU3IP1, Nop1

Background:

This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin.

UniProt Protein Function:	FBL: Involved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'- hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Interacts with NOLC1. Component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles that contain NHP2L1, FBL, NOP5 and NOP56, plus a guide RNA. It is associated with the U3, U8, U13, X and Y small nuclear RNAs. Component of several ribosomal and nucleolar protein complexes. Interacts with PRMT5 and UTP20. Interacts with DDX5. Belongs to the methyltransferase superfamily. Fibrillarin family.
UniProt Protein Details:	Protein type:EC 2.1.1.-; RNA-binding; Nucleolus; Methyltransferase; RNA processing Chromosomal Location of Human Ortholog: 19q13.1 Cellular Component: Cajal body; granular component; membrane; nucleolus; nucleus Molecular Function:protein binding; RNA binding Biological Process: osteoblast differentiation; snoRNA metabolic process; tRNA processing; rRNA processing
NCBI Summary:	This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin. [provided by RefSeq, Jul 2008]
UniProt Code:	P22087
NCBI GenInfo Identifier:	14549159
NCBI Gene ID:	2091
NCBI Accession:	P22087.2
UniProt Secondary Accession:	P22087,O75259, Q6IAT5, Q9UPI6, B5BUE8,
UniProt Related Accession:	P22087
Molecular Weight:	321
NCBI Full Name:	rRNA 2'-O-methyltransferase fibrillarin
NCBI Synonym Full Names:	fibrillarin
NCBI Official Symbol:	FBL
NCBI Official Synonym Symbols:	FIB; FLRN; RNU3IP1
NCBI Protein Information:	rRNA 2'-O-methyltransferase fibrillarin; 34-kD nucleolar scleroderma antigen; histone-glutamine methyltransferase; 34 kDa nucleolar scleroderma antigen; RNA, U3 small nucleolar interacting protein 1
UniProt Protein Name:	rRNA 2'-O-methyltransferase fibrillarin
UniProt Synonym Protein Names:	34 kDa nucleolar scleroderma antigen; Histone-glutamine methyltransferase
Protein Family:	rRNA 2'-O-methyltransferase fibrillarin
UniProt Gene Name:	FBL
UniProt Entry Name:	FBRL_HUMAN

	Western blot analysis of extracts of various cell lines, using FBL antibody (CAB13490) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded mouse liver using FBL antibody (CAB13490) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using FBL antibody (CAB13490) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of C6 cells using FBL antibody (CAB13490) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH/3T3 cells using FBL antibody (CAB13490) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-FBL Antibody (CAB1136)	Human FBLN4 / Fibrillarin ELISA Kit
Anti-Fibrillarin Antibody (CAB0850)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)