The FBP2 Antibody (CAB9850) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a gluconeogenesis regulatory enzyme which catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate and inorganic phosphate.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
FBP2 Antibody
SKU:
CAB9850
Size:
100μL, 20μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISAIF-P
Recommended Dilution:
WB
1:500 - 1:2000
IF-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CORLK, FBP2
Positive Sample:
Mouse liver, Mouse skeletal muscle, Rat skeletal muscle, Rat kidney
Cellular Localization:
Cell Junction, Cytoplasm, Nucleus, Z Line, Myofibril, Sarcomere.
Calculated MW:
37kDa
Observed MW:
36kDa
This gene encodes a gluconeogenesis regulatory enzyme which catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate and inorganic phosphate.
Purification Method
Affinity purification
Gene ID
8789
RRID
AB_2769425
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using FBP2 Rabbit pAb (CAB9850) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of paraffin-embedded Mouse skeletal muscle using FBP2 Rabbit pAb (CAB9850) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
