The FBXO3 Antibody (CAB15813) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high specificity and reactivity with human samples, making it an ideal choice for various research applications, including Western blot analysis.FBXO3 is a substrate recognition component of the Skp1-Cullin1-F-box protein (SCF) E3 ubiquitin ligase complex, playing a crucial role in the targeted degradation of specific cellular proteins. By targeting substrates for ubiquitination and subsequent degradation, FBXO3 contributes to the maintenance of cellular homeostasis and regulation of various cellular processes.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
FBXO3 Antibody
SKU:
CAB15813
Size:
20μL, 100μL
Reactivity:
Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
FBA, FBX3, FBXO3
Positive Sample:
Mouse liver, Mouse lung, Mouse eye
Cellular Localization:
Nucleus.
Calculated MW:
55kDa
Observed MW:
54kDa
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants diverging at the 3' end.
Purification Method
Affinity purification
Gene ID
26273
RRID
AB_2763236
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using FBXO3 Rabbit pAb (CAB15813) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
