Anti-FBXW5 Antibody (CAB9345)

Antibody Name:	Anti-FBXW5 Antibody
Antibody SKU:	CAB9345
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 184-566 of human FBXW5 (NP_061871.1).

Application:	WB
Recommended Dilution:	WB 1:200 - 1:1000
Reactivity:	Human
Positive Samples:	A-549

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 184-566 of human FBXW5 (NP_061871.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VRNK PYDV FGCW LTET SLIS GNLH RIGD ITSC SVLW LNNA FQDV ESEN VNVV KRLF KIQN LNAS TVRT VMVA DCSR FDSP DLLL EAGD PATS PCRI FDLG SDNE EVVA GPAP AHAK EGLR HFLD RVLE GRAQ PQLS ERML ETKV AELL AQGH TKPP ERSA TGAK SKYL IFTT GCLT YSPH QIGI KQIL PHQM TTAG PVLG EGRG SDAF FDAL DHVI DIHG HIIG MGLS PDNR YLYV NSRA WPNG AVVA DPMQ PPPI AEEI DLLV FDLK TMRE VRRA LRAH RAYT PNDE CFFI FLDV SRDF VASG AEDR HGYI WDRH YNIC LARL RHED VVNS VVFS PQEQ ELLL TASD DATI KAWR SPRT MRVL QAPR PRPR TFFS WLAS QRR
Gene ID:	54461
Uniprot:	Q969U6
Cellular Location:	Cytoplasm
Calculated MW:	42kDa/63kDa
Observed MW:	72kDa

Synonyms:

FBXW5, Fbw5

Background:

This gene encodes a member of the F-box protein family, members of which are characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into three classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene contains WD-40 domains, in addition to an F-box motif, so it belongs to the Fbw class. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene, however, they were found to be nonsense-mediated mRNA decay (NMD) candidates, hence not represented.

UniProt Protein Function:	FBXW5: Substrate recognition component of both SCF (SKP1-CUL1- F-box protein) and DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes. Substrate recognition component of the SCF(FBXW5) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of SASS6 during S phase, leading to prevent centriole reduplication. Substrate-specific adapter of the DCX(FBXW5) E3 ubiquitin-protein ligase complex which mediates the polyubiquitination and subsequent degradation of TSC2. May also act as a negative regulator of MAP3K7/TAK1 signaling in the interleukin-1B (IL1B) signaling pathway. Belongs to the FBXW5 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 9q34.3 Cellular Component: cytoplasm; SCF ubiquitin ligase complex Molecular Function:protein binding; protein kinase binding Biological Process: centrosome duplication; proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
NCBI Summary:	This gene encodes a member of the F-box protein family, members of which are characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into three classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene contains WD-40 domains, in addition to an F-box motif, so it belongs to the Fbw class. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene, however, they were found to be nonsense-mediated mRNA decay (NMD) candidates, hence not represented. [provided by RefSeq, Jul 2008]
UniProt Code:	Q969U6
NCBI GenInfo Identifier:	44887886
NCBI Gene ID:	54461
NCBI Accession:	Q969U6.1
UniProt Secondary Accession:	Q969U6,Q59ET5, Q5SPZ8, Q5SPZ9, Q5SQ00, Q5SQ02, Q5SQ03 Q5SQ04, Q8WY79, Q96GJ6, Q9BSU8, B2RDZ6,
UniProt Related Accession:	Q969U6
Molecular Weight:	42,185 Da
NCBI Full Name:	F-box/WD repeat-containing protein 5
NCBI Synonym Full Names:	F-box and WD repeat domain containing 5
NCBI Official Symbol:	FBXW5
NCBI Official Synonym Symbols:	Fbw5
NCBI Protein Information:	F-box/WD repeat-containing protein 5
UniProt Protein Name:	F-box/WD repeat-containing protein 5
UniProt Synonym Protein Names:	F-box and WD-40 domain-containing protein 5
Protein Family:	F-box/WD repeat-containing protein
UniProt Gene Name:	FBXW5
UniProt Entry Name:	FBXW5_HUMAN

Anti-FBXW5 Polyclonal Antibody (CAB9345)

Western blot analysis of extracts of A-549 cells, using FBXW5 antibody (CAB9345) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

Antibodies

Anti-FBXW5 Antibody (CAB16694)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)