Description
FKHRL1 (Phospho-Ser253)Transcription Factor Activity Assay
The FKHR/L1 Phospho-Ser253 Transcription Factor Activity Assay is a powerful tool for studying the activity of this key transcription factor in cellular processes. With high sensitivity and specificity, this assay provides accurate and reproducible results, making it an invaluable asset for researchers in the field.FKHR/L1, also known as FOXO3, is a transcription factor that plays a critical role in regulating various cellular functions, including cell cycle progression, apoptosis, and oxidative stress response. Phosphorylation of Ser253 is known to modulate its activity, making this assay particularly useful in understanding the regulatory mechanisms of FKHR/L1.
This assay kit is designed for use with cell lysates and provides a convenient and efficient method for quantifying FKHR/L1 phosphorylation at Ser253. By accurately measuring the activity of this transcription factor, researchers can gain insights into its role in various physiological and pathological processes, including aging, cancer, and metabolic disorders.
| Product Name: | FKHRL1 (Phospho-Ser253)Transcription Factor Activity Assay |
| Product Code: | TFAB00028 |
| Target: | FKHRL1 (Phospho-Ser253) |
| Synonyms: | AF6q21 protein, Forkhead in rhabdomyosarcoma-like 1 |
| Reactivity: | Human, Mouse |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie FKHRL1 (Phospho-Ser253) transcription factor activity assay allows for the detection and qualitative analysis of FKHRL1 (Phospho-Ser253) active transcription factors in eukaryotic nuclear or cell lysates.
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | FOXO3A: a transcription factors of the forkhead family. Contains 1 fork-head domain. May regulate apoptosis through control of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. |
| UniProt Protein Details: | Protein type:Oncoprotein; DNA-binding; Transcription factor; Autophagy Chromosomal Location of Human Ortholog: 6q21 Cellular Component: nucleoplasm; membrane; cytoplasm; cytosol; nucleus Molecular Function:protein binding; DNA binding; chromatin DNA binding; sequence-specific DNA binding; transcription factor activity; protein kinase binding Biological Process: epidermal growth factor receptor signaling pathway; transcription from RNA polymerase II promoter; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; DNA damage response, signal transduction by p53 class mediator; nerve growth factor receptor signaling pathway; initiation of primordial ovarian follicle growth; positive regulation of transcription, DNA-dependent; antral ovarian follicle growth; positive regulation of erythrocyte differentiation; negative regulation of transcription from RNA polymerase II promoter; glucose homeostasis; ovulation from ovarian follicle; oocyte maturation; regulation of translation; regulation of transcription from RNA polymerase II promoter; positive regulation of neuron apoptosis; innate immune response; positive regulation of transcription from RNA polymerase II promoter |
| NCBI Summary: | This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O43524 |
| NCBI GenInfo Identifier: | 8134467 |
| NCBI Gene ID: | 2309 |
| NCBI Accession: | O43524.1 |
| UniProt Secondary Accession: | O43524,O15171, Q5T2I7, Q9BZ04, B4DVZ6, E1P5E6, |
| UniProt Related Accession: | O43524 |
| Molecular Weight: | |
| NCBI Full Name: | Forkhead box protein O3 |
| NCBI Synonym Full Names: | forkhead box O3 |
| NCBI Official Symbol: | FOXO3 |
| NCBI Official Synonym Symbols: | FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2 |
| NCBI Protein Information: | forkhead box protein O3; forkhead box O3A; forkhead in rhabdomyosarcoma-like 1; forkhead homolog (rhabdomyosarcoma) like 1; forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1 |
| UniProt Protein Name: | Forkhead box protein O3 |
| UniProt Synonym Protein Names: | AF6q21 protein; Forkhead in rhabdomyosarcoma-like 1 |
| Protein Family: | Forkhead box protein |
| UniProt Gene Name: | FOXO3 |
| UniProt Entry Name: | FOXO3_HUMAN |
