Anti-FLNB Antibody (CAB2481)

Antibody Name:	Anti-FLNB Antibody
Antibody SKU:	CAB2481
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1686-1785 of human FLNB (NP_001157789.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:50 - 1:100 IF 1:20 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, NIH/3T3, A-431, Mouse liver, Mouse kidney, Mouse uterus, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1686-1785 of human FLNB (NP_001157789.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PDGT EAEA DVIE NEDG TYDI FYTA AKPG TYVI YVRF GGVD IPNS PFTV MATD GEVT AVEE APVN ACPP GFRP WVTE EAYV PVSD MNGL GFKP FDLV IPFA
Gene ID:	2317
Uniprot:	O75369
Cellular Location:	Cytoplasm, Z line, cell cortex, cytoskeleton, myofibril, sarcomere
Calculated MW:	230-281kDa
Observed MW:	298kDa

Synonyms:

FLNB, ABP-278, ABP-280, AOI, FH1, FLN-B, FLN1L, LRS1, SCT, TABP, TAP, filamin-B

Background:

This gene encodes a member of the filamin family. The encoded protein interacts with glycoprotein Ib alpha as part of the process to repair vascular injuries. The platelet glycoprotein Ib complex includes glycoprotein Ib alpha, and it binds the actin cytoskeleton. Mutations in this gene have been found in several conditions: atelosteogenesis type 1 and type 3; boomerang dysplasia; autosomal dominant Larsen syndrome; and spondylocarpotarsal synostosis syndrome. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene.

UniProt Protein Function:	FLNB: a ubiquitous cytoskeletal protein that links actin filaments to membrane glycoproteins. Six alternatively spliced isoforms have been described. Various interactions and localizations of isoforms affect myotube morphology and myogenesis. Isoforms 1 and 2 are expressed in placenta, bone marrow, brain, umbilical vein endothelial cells, retina and skeletal muscle. Isoform 1 is predominantly expressed in prostate, uterus, liver, thyroid, stomach, lymph node, small intestine, spleen, skeletal muscle, kidney, placenta, pancreas, heart, lung, platelets, endothelial cells, megakaryocytic and erythroleukemic cell lines. Isoform 2 is predominantly expressed in spinal cord and platelets. Also expressed in thyroid adenoma, neurofibrillary tangles (NFT), senile plaques in the hippocampus and cerebral cortex in Alzheimer's disease. Isoform 3 and isoform 6 are expressed predominantly in lung, heart, skeletal muscle, testis, spleen, thymus and leukocytes. Isoform 4 and isoform 5 are expressed in heart.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Actin-binding Chromosomal Location of Human Ortholog: 3p14.3 Cellular Component: focal adhesion; cytoplasm; integral to membrane; stress fiber; plasma membrane; cell cortex; Z disc; cytosol; actin cytoskeleton Molecular Function:identical protein binding; protein binding; actin binding Biological Process: skeletal muscle development; cytoskeletal anchoring; cytokine and chemokine mediated signaling pathway; actin cytoskeleton organization and biogenesis; signal transduction; cell differentiation Disease: Larsen Syndrome; Boomerang Dysplasia; Atelosteogenesis, Type I; Spondylocarpotarsal Synostosis Syndrome; Atelosteogenesis, Type Iii
NCBI Summary:	This gene encodes a member of the filamin family. The encoded protein interacts with glycoprotein Ib alpha as part of the process to repair vascular injuries. The platelet glycoprotein Ib complex includes glycoprotein Ib alpha, and it binds the actin cytoskeleton. Mutations in this gene have been found in several conditions: atelosteogenesis type 1 and type 3; boomerang dysplasia; autosomal dominant Larsen syndrome; and spondylocarpotarsal synostosis syndrome. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Nov 2009]
UniProt Code:	O75369
NCBI GenInfo Identifier:	296434507
NCBI Gene ID:	2317
NCBI Accession:	O75369.2
UniProt Secondary Accession:	O75369,Q13706, Q59EC2, Q60FE7, Q6MZJ1, Q8WXS9, Q8WXT0 B2ZZ83, B2ZZ84, B2ZZ85, C9JKE6, C9JMC4,
UniProt Related Accession:	O75369
Molecular Weight:	2602
NCBI Full Name:	Filamin-B
NCBI Synonym Full Names:	filamin B, beta
NCBI Official Symbol:	FLNB
NCBI Official Synonym Symbols:	AOI; FH1; SCT; TAP; LRS1; TABP; FLN-B; FLN1L; ABP-278; ABP-280
NCBI Protein Information:	filamin-B; filamin-3; beta-filamin; ABP-280 homolog; filamin homolog 1; thyroid autoantigen; actin binding protein 278; actin-binding-like protein; Larsen syndrome 1 (autosomal dominant)
UniProt Protein Name:	Filamin-B
UniProt Synonym Protein Names:	ABP-278; ABP-280 homolog; Actin-binding-like protein; Beta-filamin; Filamin homolog 1; Fh1; Filamin-3; Thyroid autoantigen; Truncated actin-binding protein; Truncated ABP
Protein Family:	Filamin
UniProt Gene Name:	FLNB
UniProt Entry Name:	FLNB_HUMAN

	Western blot analysis of extracts of various cell lines, using FLNB antibody (CAB2481) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit (RM00020)._Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded rat heart using FLNB antibody (CAB2481) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver damage using FLNB antibody (CAB2481) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human prostate using FLNB antibody (CAB2481) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using FLNB antibody (CAB2481) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
FLNB Antibody (PACO09315)	Human Filamin-B ELISA Kit
	Human FLN beta (Filamin B, Beta) CLIA Kit (HUES01117)
	Human Filamin-B (FLNB) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)