The FST Antibody (CAB2936) is a high-quality antibody developed for reliable detection and analysis of target proteins. Raised in rabbits, this antibody exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the Follistatin protein, this antibody enables the detection and analysis of Follistatin in various cell types, making it ideal for studies in developmental biology, reproductive health, and muscle growth research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
FST Antibody
SKU:
CAB2936
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
AACS SGVL LEVK HSGS CNSI SEDT EEEE EDED QDYS FPIS SILE W
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:100 - 1:500
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
FS, FST
Positive Sample:
HepG2, Rat pancreas
Cellular Localization:
Secreted.
Calculated MW:
38kDa
Observed MW:
38kDa
Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. In a study in which 37 candidate genes were tested for linkage and association with polycystic ovary syndrome (PCOS) or hyperandrogenemia in 150 families, evidence was found for linkage between PCOS and follistatin.
Purification Method
Affinity purification
Gene ID
10468
RRID
AB_2764750
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using FST Rabbit pAb (CAB2936) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
